נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Ovulation-selective genes: The generation and characterization of an ovulatory-selective cDNA library
Year:
2006
Source of publication :
Journal of Endocrinology
Authors :
גרשון, ערן
;
.
Volume :
188
Co-Authors:
Hourvitz, A., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States, Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Gershon, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Hennebold, J.D., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States, Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, United States
Elizur, S., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Maman, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Brendle, C., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States
Adashi, E.Y., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States
Dekel, N., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
531
To page:
548
(
Total pages:
18
)
Abstract:
Ovulation-selective/specific genes, that is, genes preferentially or exclusively expressed during the ovulatory process, have been the subject of growing interest. We report herein studies on the use of suppression subtractive hybridization (SSH) to construct a 'forward' ovulation-selective/specific cDNA library. In toto, 485 clones were sequenced and analyzed for homology to known genes with the basic local alignment tool (BLAST). Of those, 252 were determined to be nonredundant. Of these 252 nonredundant clones, 98 were analyzed by probing mouse preovulatory and postovulatory ovarian cDNA. Twenty-five clones (26%) failed to show any signal, and 43 cDNAs tested thus far display a true ovulation-selective/specific expression pattern. In this communication, we focus on one such ovulation-selective gene, the fatty acid elongase 1 (FAE-1) homolog, found to be localized to the inner periantral granulosa and to the cumulus granulosa cells of antral follicles. The FAE-1 gene is a β-ketoacyl-CoA synthase belonging to the fatty acid elongase (ELO) family, which catalyzes the initial step of very long-chain fatty acid synthesis. All in all, the present study accomplished systematic identification of those hormonally regulated genes that are expressed in the ovary in an ovulation-selective/specific manner. These ovulation-selective/specific genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for both the promotion of fertility and its control. © 2006 Society for Endocrinology.
Note:
Related Files :
animal experiment
Animals
animal tissue
Female
Granulosa Cells
mice
עוד תגיות
תוכן קשור
More details
DOI :
10.1677/joe.1.06231
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23653
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:01
Scientific Publication
Ovulation-selective genes: The generation and characterization of an ovulatory-selective cDNA library
188
Hourvitz, A., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States, Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Gershon, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Hennebold, J.D., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States, Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, OR 97006, United States
Elizur, S., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Maman, E., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Brendle, C., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States
Adashi, E.Y., Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Utah Health Sciences Center, Salt Lake City, UT 84132, United States
Dekel, N., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel
Ovulation-selective genes: The generation and characterization of an ovulatory-selective cDNA library
Ovulation-selective/specific genes, that is, genes preferentially or exclusively expressed during the ovulatory process, have been the subject of growing interest. We report herein studies on the use of suppression subtractive hybridization (SSH) to construct a 'forward' ovulation-selective/specific cDNA library. In toto, 485 clones were sequenced and analyzed for homology to known genes with the basic local alignment tool (BLAST). Of those, 252 were determined to be nonredundant. Of these 252 nonredundant clones, 98 were analyzed by probing mouse preovulatory and postovulatory ovarian cDNA. Twenty-five clones (26%) failed to show any signal, and 43 cDNAs tested thus far display a true ovulation-selective/specific expression pattern. In this communication, we focus on one such ovulation-selective gene, the fatty acid elongase 1 (FAE-1) homolog, found to be localized to the inner periantral granulosa and to the cumulus granulosa cells of antral follicles. The FAE-1 gene is a β-ketoacyl-CoA synthase belonging to the fatty acid elongase (ELO) family, which catalyzes the initial step of very long-chain fatty acid synthesis. All in all, the present study accomplished systematic identification of those hormonally regulated genes that are expressed in the ovary in an ovulation-selective/specific manner. These ovulation-selective/specific genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for both the promotion of fertility and its control. © 2006 Society for Endocrinology.
Scientific Publication
You may also be interested in