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פותח על ידי קלירמאש פתרונות בע"מ -
NADP+-isocitrate dehydrogenase gene expression and isozyme activity during citrus fruit development
Year:
2000
Source of publication :
Plant Science
Authors :
אור, אתי
;
.
דהן, אסתר
;
.
צדקה, אבי
;
.
Volume :
158
Co-Authors:
Sadka, A., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Dahan, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Or, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cohen, L., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
173
To page:
181
(
Total pages:
9
)
Abstract:
The accumulation of citric acid and its decline toward fruit maturation is typical of citrus fruit. We studied NADP+-isocitrate dehydrogenase (NADP-IDH), an enzyme involved in citrate metabolism. A cDNA encoding the enzyme was cloned from lemon (Citrus limon) juice sac cells, and is the first-reported NADP-IDH from fruits. Sequence comparisons and phylogenetic analysis indicate that it most probably belongs to a monophyletic clade of plant cytosolic enzymes. The mRNA level in the juice sac cells was induced during lemon fruit growth, and increased by about 15-fold to a peak as the fruit neared maturation. Spectrophotometric assay of the NADP-IDH activity in the pulp during fruit development showed that in young fruit, most of the activity was associated with the mitochondrial preparation and that, as the fruit grew, the activity shifted to the soluble fraction. The two activities could also be distinguished by isozyme gel electrophoresis: while one isozyme was detected in the mitochondrial preparation of young fruit and declined later, the other was induced in the soluble fraction of older fruit and increased as the fruit grew. The increasing activity of NADP-IDH in the soluble fraction throughout fruit development correlated well with the increase in gene expression, which suggests that the soluble activity is regulated by the expression of the cytosolic NADP-IDH gene. The possible role of this form of the enzyme in citric acid catabolism in the pulp is discussed. (C) 2000 Elsevier Science Ireland Ltd.
Note:
Related Files :
Citrus limon
enzyme activity
gene expression
isoenzyme
metabolism
NADP+-isocitrate dehydrogenase
plant growth
sequence analysis
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/S0168-9452(00)00328-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23671
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:01
You may also be interested in
Scientific Publication
NADP+-isocitrate dehydrogenase gene expression and isozyme activity during citrus fruit development
158
Sadka, A., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Dahan, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Or, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cohen, L., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
NADP+-isocitrate dehydrogenase gene expression and isozyme activity during citrus fruit development
The accumulation of citric acid and its decline toward fruit maturation is typical of citrus fruit. We studied NADP+-isocitrate dehydrogenase (NADP-IDH), an enzyme involved in citrate metabolism. A cDNA encoding the enzyme was cloned from lemon (Citrus limon) juice sac cells, and is the first-reported NADP-IDH from fruits. Sequence comparisons and phylogenetic analysis indicate that it most probably belongs to a monophyletic clade of plant cytosolic enzymes. The mRNA level in the juice sac cells was induced during lemon fruit growth, and increased by about 15-fold to a peak as the fruit neared maturation. Spectrophotometric assay of the NADP-IDH activity in the pulp during fruit development showed that in young fruit, most of the activity was associated with the mitochondrial preparation and that, as the fruit grew, the activity shifted to the soluble fraction. The two activities could also be distinguished by isozyme gel electrophoresis: while one isozyme was detected in the mitochondrial preparation of young fruit and declined later, the other was induced in the soluble fraction of older fruit and increased as the fruit grew. The increasing activity of NADP-IDH in the soluble fraction throughout fruit development correlated well with the increase in gene expression, which suggests that the soluble activity is regulated by the expression of the cytosolic NADP-IDH gene. The possible role of this form of the enzyme in citric acid catabolism in the pulp is discussed. (C) 2000 Elsevier Science Ireland Ltd.
Scientific Publication
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