LERER, M., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
BAR‐AKIVA, A., Agricultural Research Organization, Volcani Center, Bet Dagan, Israel

Total (Kjeldahl) nitrogen content of manganese‐deficient lemon leaves was found to be about 40% higher (on a dry weight basis) than in the analog control leaves. The nitrate‐N content rose in the deficient leaves from 18.2 mg/100 g to 95.6 mg/100 g dry weight. Among the N constituents studied, the free amino acids increased by about 42%, the protein amino acids by about 22%, and the total protein by more than 22%, all on a fresh weight basis. Acrylamide gel electrophoresis and ammonium sulfate fractionation showed qualitative differences in the various proteins. The increase of the protein fraction in the ‐Mn leaves may be attributed in part to the decrease of dry matter in these leaves (25.8% as compared to 30.7% in the control leaves) and in part to accumulation of apparent proteinous material in manganese‐deficient tissues. Copyright © 1976, Wiley Blackwell. All rights reserved