חיפוש מתקדם
Plant Molecular Biology
Sessa, G., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Yang, X.-Q., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Raz, V., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Eyal, Y., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
The PRB-1b gene codes for a basic-type pathogenesis-related protein of the PR-1 family of tobacco. PRB-1b mRNA accumulation is induced in response to biotic and abiotic elicitors, such as TMV, ethylene, salicylic acid, α-amino butyric acid and darkness. In order to determine the location of elements that control dark-regulated PRB-1b gene expression, we tested promoter, transcribed regions and 3′-downstream regions of the gene for their ability to respond to dark induction in transgenic tobacco plants. An ethylene-inducible promoter region of 863 bp was not able to confer dark induction to a β-glucuronidase reporter, gene, while a construct containing the transcribed region of the gene and 3′-downstream sequences, driven by the cauliflower mosaic virus 35S promoter, was correctly dark-regulated. The results indicate that dark-induction of the PRB-1b gene can be controlled by 3′-downstream elements at the transcriptional level or by transcribed sequences at the post-transcriptional level. A circadian clock regulation of the PRB-1b gene was excluded, as fluctuations of PRB-1b transcript levels were not observed in plants placed in constant light or darkness. Subcellular localization of the PRB-1b protein was also determined, in tobacco protoplasts preparations and in cell cultures. The PRB-1b polypeptide was predominantly detected in protoplast vacuoles and was not secreted to the media in cell cultures. These results support an intracellular localization for the PRB-1b protein, as reported for other basic-type components of the pathogenesis-related proteins family. © 1995 Kluwer Academic Publishers.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Dark induction and subcellular localization of the pathogenesis-related PRB-1b protein
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Sessa, G., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Yang, X.-Q., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Raz, V., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Eyal, Y., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Fluhr, R., Department of Plant Genetics, Weizmann Institute of Science, P.O. Box 26, Rehovot, 76100, Israel
Dark induction and subcellular localization of the pathogenesis-related PRB-1b protein
The PRB-1b gene codes for a basic-type pathogenesis-related protein of the PR-1 family of tobacco. PRB-1b mRNA accumulation is induced in response to biotic and abiotic elicitors, such as TMV, ethylene, salicylic acid, α-amino butyric acid and darkness. In order to determine the location of elements that control dark-regulated PRB-1b gene expression, we tested promoter, transcribed regions and 3′-downstream regions of the gene for their ability to respond to dark induction in transgenic tobacco plants. An ethylene-inducible promoter region of 863 bp was not able to confer dark induction to a β-glucuronidase reporter, gene, while a construct containing the transcribed region of the gene and 3′-downstream sequences, driven by the cauliflower mosaic virus 35S promoter, was correctly dark-regulated. The results indicate that dark-induction of the PRB-1b gene can be controlled by 3′-downstream elements at the transcriptional level or by transcribed sequences at the post-transcriptional level. A circadian clock regulation of the PRB-1b gene was excluded, as fluctuations of PRB-1b transcript levels were not observed in plants placed in constant light or darkness. Subcellular localization of the PRB-1b protein was also determined, in tobacco protoplasts preparations and in cell cultures. The PRB-1b polypeptide was predominantly detected in protoplast vacuoles and was not secreted to the media in cell cultures. These results support an intracellular localization for the PRB-1b protein, as reported for other basic-type components of the pathogenesis-related proteins family. © 1995 Kluwer Academic Publishers.
Scientific Publication
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