Co-Authors:
Sahly, H., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany, Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel, Dept. of Med. Microbiol. and Virol., University of Kiel, Brunswiker Str. 4, 24105 Kiel, Germany
Podschun, R., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Oelschlaeger, T.A., Inst. fur Molec. Infektionsbiologie, University of Würzburg, Würzburg, Germany
Greiwe, M., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Parolis, H., School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa
Hasty, D., VA Medical Center, Dept. of Anatomy and Neurobiology, University of Tennessee, Memphis, TN, United States
Kekow, J., Clinic of Rheumatology, University of Magdeburg, Vogelsang, Germany
Ullmann, U., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Ofek, I., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Sela, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Abstract:
The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.
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