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פותח על ידי קלירמאש פתרונות בע"מ -
Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae
Year:
2000
Source of publication :
Infection and Immunity
Authors :
סלע, שלמה
;
.
Volume :
68
Co-Authors:
Sahly, H., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany, Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel, Dept. of Med. Microbiol. and Virol., University of Kiel, Brunswiker Str. 4, 24105 Kiel, Germany
Podschun, R., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Oelschlaeger, T.A., Inst. fur Molec. Infektionsbiologie, University of Würzburg, Würzburg, Germany
Greiwe, M., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Parolis, H., School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa
Hasty, D., VA Medical Center, Dept. of Anatomy and Neurobiology, University of Tennessee, Memphis, TN, United States
Kekow, J., Clinic of Rheumatology, University of Magdeburg, Vogelsang, Germany
Ullmann, U., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Ofek, I., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Sela, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Facilitators :
From page:
6744
To page:
6749
(
Total pages:
6
)
Abstract:
The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.
Note:
Related Files :
bacterial membrane
bacterium adherence
cell line
human
human cell
Intestinal Mucosa
klebsiella pneumoniae
עוד תגיות
תוכן קשור
More details
DOI :
10.1128/IAI.68.12.6744-6749.2000
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23699
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:01
Scientific Publication
Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae
68
Sahly, H., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany, Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel, Dept. of Med. Microbiol. and Virol., University of Kiel, Brunswiker Str. 4, 24105 Kiel, Germany
Podschun, R., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Oelschlaeger, T.A., Inst. fur Molec. Infektionsbiologie, University of Würzburg, Würzburg, Germany
Greiwe, M., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Parolis, H., School of Pharmaceutical Sciences, Rhodes University, Grahamstown, South Africa
Hasty, D., VA Medical Center, Dept. of Anatomy and Neurobiology, University of Tennessee, Memphis, TN, United States
Kekow, J., Clinic of Rheumatology, University of Magdeburg, Vogelsang, Germany
Ullmann, U., Dept. of Med. Microbiol. and Virol., University of Kiel, Kiel, Germany
Ofek, I., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Sela, S., Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel
Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae
The adhesion of K21a, K26, K36, and K50 capsulated Klebsiella strains to ileocecal (HCT-8) and bladder (T24) epithelial cell lines was significantly lower than that of their corresponding spontaneous noncapsulated variants K21a/3, K26/1, K36/3, and K50/3, respectively. Internalization of the bacteria by both epithelial cell lines was also significantly reduced. Similarly, a capsule-switched derivative, K2(K36), that exhibited a morphologically larger K36 capsule and formed more capsular material invaded the ileocecal epithelial cell line poorly compared to the corresponding K2 parent strain. None of the capsulated strains exhibited significant mannose-sensitive type 1 fimbriae, whereas two of the noncapsulated variants K21a/3 and K50/3 exhibited potent mannose-sensitive hemagglutinating activity. Although hemagglutinating activity that could be attributed to mannose-resistant Klebsiella type 3 fimbriae was weak in all strains, in several cases the encapsulated parent strains exhibited lower titers than their corresponding noncapsulated variants. Although the level of adhesion to the ileocecal cells is not different from adhesion to bladder cells, bacterial internalization by bladder cells was significantly lower than internalization by ileocecal cells, suggesting that bladder cells lack components required for the internalization of Klebsiella.
Scientific Publication
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