Booster immunization with a partially purified citrus tristeza virus (CTV) preparation after priming with recombinant CTV coat protein enhances the binding capacity of capture antibodies by ELISA

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פותח על ידי קלירמאש פתרונות בע"מ -

Booster immunization with a partially purified citrus tristeza virus (CTV) preparation after priming with recombinant CTV coat protein enhances the binding capacity of capture antibodies by ELISA

Year: 

1997

Source of publication :

Journal of Virological Methods

Authors :

בר-יוסף, משה

;

גוטויין, אלישע

;

גופמן, רוז

;

יאנג, גואנג

;

מוואסי, מוניר

.

Volume :

Co-Authors: 

Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Filatov, V., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Gofman, R., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Guang, Y., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Hadjinicolis, A., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Bet Dagan 50250, Israel
Gootwine, E., Animal Reproduction, Bet Dagan 50250, Israel, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Weisman, Y., Div. of Avian and Fish Dis. K.V.I., Bet Dagan 50250, Israel
Malkinson, M., Div. of Avian and Fish Dis. K.V.I., Bet Dagan 50250, Israel

From page: 

19

To page: 

22

(

Total pages: 

4

)

Link :

Booster immunization with a partially purified citrus tristeza virus (CTV) preparation after priming with recombinant CTV coat protein enhances the binding capacity of capture antibodies by ELISA

Abstract: 

Groups of rabbits and young lambs were immunized subcutaneously and intramuscularly with a recombinant citrus tristeza virus (CTV) coat protein (rCTV-CP) antigen. Three weeks after primary immunization the animals were divided into two groups that were boosted either with rCTV-CP or with a partially purified preparation of CTV particles (ppCTV). Twelve and 15 days after the last injection, the animals were bled and the binding capacity of the antisera for CTV detection was examined for capture antibodies by the indirect ELISA. Considerably higher ELISA titers were obtained from animals that were boosted with ppCTV than with rCP. Boosting with partially purified native antigens after priming with recombinant antigens is expected to extend the applicability of the antisera for detecting other structural and non- structural viral antigens by trapping ELISA.