Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans

שלום

עברית

מדריך מקוצר למשתמש מדריך מפורט למשתמש

חפש...

הספר "אוצר וולקני"

אודות

תנאי שימוש

פותח על ידי קלירמאש פתרונות בע"מ -

Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans

Year: 

2011

Source of publication :

Microbiology

Authors :

גאבה, ויקטור

;

מנוליס-ששון, שולמית

.

Volume :

157

Co-Authors: 

Weinthal, D.M., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel, Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Tzfira, T., Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Gaba, V., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel

From page: 

1500

To page: 

1508

(

Total pages: 

9

)

Link :

Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans

Abstract: 

HsvG and HsvB, two paralogous type III effectors of the gall-forming bacteria Pantoea agglomerans pv. gypsophilae and P. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. They were previously shown to be DNA-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. Sequence analysis of these effectors did not detect canonical nuclear localization signals (NLSs), but two basic amino acid clusters designated putative NLS1 and NLS2 were detected in their N-terminal and C-terminal regions, respectively. pNIA assay for nuclear import in yeast and bombardment of melon leaves with each of the NLSs fused to a 2xYFP reporter indicated that putative NLS1 and NLS2 were functional in transport of HsvG into the nucleus. A yeast two-hybrid assay showed that HsvB, HsvG, putative NLS1, putative NLS2, HsvG converted into HsvB, or HsvB converted into HsvG by exchanging the repeat domain, all interacted with AtKAP-α and importin-α3 of Arabidopsis thaliana. Deletion analysis of the NLS domains in HsvG suggested that putative NLS1 or NLS2 were required for pathogenicity on gypsophila cuttings and presumably for import of HsvG into the nucleus. This study demonstrates the presence of two functional NLSs in the type III effectors HsvG and HsvB. © 2011 SGM.