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Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans
Year:
2011
Source of publication :
Microbiology
Authors :
גאבה, ויקטור
;
.
מנוליס-ששון, שולמית
;
.
Volume :
157
Co-Authors:
Weinthal, D.M., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel, Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Tzfira, T., Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Gaba, V., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
1500
To page:
1508
(
Total pages:
9
)
Abstract:
HsvG and HsvB, two paralogous type III effectors of the gall-forming bacteria Pantoea agglomerans pv. gypsophilae and P. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. They were previously shown to be DNA-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. Sequence analysis of these effectors did not detect canonical nuclear localization signals (NLSs), but two basic amino acid clusters designated putative NLS1 and NLS2 were detected in their N-terminal and C-terminal regions, respectively. pNIA assay for nuclear import in yeast and bombardment of melon leaves with each of the NLSs fused to a 2xYFP reporter indicated that putative NLS1 and NLS2 were functional in transport of HsvG into the nucleus. A yeast two-hybrid assay showed that HsvB, HsvG, putative NLS1, putative NLS2, HsvG converted into HsvB, or HsvB converted into HsvG by exchanging the repeat domain, all interacted with AtKAP-α and importin-α3 of Arabidopsis thaliana. Deletion analysis of the NLS domains in HsvG suggested that putative NLS1 or NLS2 were required for pathogenicity on gypsophila cuttings and presumably for import of HsvG into the nucleus. This study demonstrates the presence of two functional NLSs in the type III effectors HsvG and HsvB. © 2011 SGM.
Note:
Related Files :
Beta vulgaris
carboxy terminal sequence
Gypsophila
Yeast
עוד תגיות
תוכן קשור
More details
DOI :
10.1099/mic.0.047118-0
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23731
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:02
You may also be interested in
Scientific Publication
Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans
157
Weinthal, D.M., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel, Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Tzfira, T., Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States
Gaba, V., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, The Volcani Center, Bet Dagan 50250, Israel
Characterization of nuclear localization signals in the type III effectors HsvG and HsvB of the gallforming bacterium Pantoea agglomerans
HsvG and HsvB, two paralogous type III effectors of the gall-forming bacteria Pantoea agglomerans pv. gypsophilae and P. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. They were previously shown to be DNA-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. Sequence analysis of these effectors did not detect canonical nuclear localization signals (NLSs), but two basic amino acid clusters designated putative NLS1 and NLS2 were detected in their N-terminal and C-terminal regions, respectively. pNIA assay for nuclear import in yeast and bombardment of melon leaves with each of the NLSs fused to a 2xYFP reporter indicated that putative NLS1 and NLS2 were functional in transport of HsvG into the nucleus. A yeast two-hybrid assay showed that HsvB, HsvG, putative NLS1, putative NLS2, HsvG converted into HsvB, or HsvB converted into HsvG by exchanging the repeat domain, all interacted with AtKAP-α and importin-α3 of Arabidopsis thaliana. Deletion analysis of the NLS domains in HsvG suggested that putative NLS1 or NLS2 were required for pathogenicity on gypsophila cuttings and presumably for import of HsvG into the nucleus. This study demonstrates the presence of two functional NLSs in the type III effectors HsvG and HsvB. © 2011 SGM.
Scientific Publication
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