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Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development
Year:
2008
Authors :
מנוליס-ששון, שולמית
;
.
Volume :
146
Co-Authors:
Balaji, V., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Mayrose, M., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Sherf, O., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Jacob-Hirsch, J., Department of Pediatric Hemato-Oncology, Safra Children's Hospital, Cancer Research Center, Tel Hashomer 52621, Israel
Eichenlaub, R., Department of Genetechnology/Microbiology, University of Bielefeld, D-33615 Bielefeld, Germany
Iraki, N., United Nations Educational, Scientific and Cultural Organization Biotechnology Center, Bethlehem University, Bethlehem, Palestine
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rechavi, G., Department of Pediatric Hemato-Oncology, Safra Children's Hospital, Cancer Research Center, Tel Hashomer 52621, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Sessa, G., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
1797
To page:
1809
(
Total pages:
13
)
Abstract:
Clavibacter michiganensis subsp. michiganensis (Cmm) is a gram-positive actinomycete, causing bacterial wilt and canker disease in tomato (Solanum lycopersicum). Host responses to gram-positive bacteria and molecular mechanisms associated with the development of disease symptoms caused by Cmm in tomato are largely unexplored. To investigate plant responses activated during this compatible interaction, we used microarray analysis to monitor changes in host gene expression during disease development. This analysis was performed at 4 d postinoculation, when bacteria were actively multiplying and no wilt symptoms were yet visible; and at 8 d postinoculation, when bacterial growth approached saturation and typical wilt symptoms were observed. Of the 9,254 tomato genes represented on the array, 122 were differentially expressed in Cmminfected plants, compared with mock-inoculated plants. Functional classification of Cmm-responsive genes revealed that Cmm activated typical basal defense responses in the host, including induction of defense-related genes, production and scavenging of free oxygen radicals, enhanced protein turnover, and hormone synthesis. Cmm infection also induced a subset of host genes involved in ethylene biosynthesis and response. After inoculation with Cmm, Never ripe (Nr) mutant plants, impaired in ethylene perception, and transgenic plants with reduced ethylene synthesis showed significant delay in the appearance of wilt symptoms, compared with wild-type plants. The retarded wilting in Nr plants was a specific effect of ethylene insensitivity, and was not due to altered expression of defense-related genes, reduced bacterial populations, or decreased ethylene synthesis. Taken together, our results indicate that host-derived ethylene plays an important role in regulation of the tomato susceptible response to Cmm. © 2008 American Society of Plant Biologists.
Note:
Related Files :
ethylene
Genetics
Growth, Development and Aging
metabolism
Solanum
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.107.115188
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23781
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:02
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Scientific Publication
Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development
146
Balaji, V., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Mayrose, M., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Sherf, O., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Jacob-Hirsch, J., Department of Pediatric Hemato-Oncology, Safra Children's Hospital, Cancer Research Center, Tel Hashomer 52621, Israel
Eichenlaub, R., Department of Genetechnology/Microbiology, University of Bielefeld, D-33615 Bielefeld, Germany
Iraki, N., United Nations Educational, Scientific and Cultural Organization Biotechnology Center, Bethlehem University, Bethlehem, Palestine
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Rechavi, G., Department of Pediatric Hemato-Oncology, Safra Children's Hospital, Cancer Research Center, Tel Hashomer 52621, Israel
Barash, I., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Sessa, G., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development
Clavibacter michiganensis subsp. michiganensis (Cmm) is a gram-positive actinomycete, causing bacterial wilt and canker disease in tomato (Solanum lycopersicum). Host responses to gram-positive bacteria and molecular mechanisms associated with the development of disease symptoms caused by Cmm in tomato are largely unexplored. To investigate plant responses activated during this compatible interaction, we used microarray analysis to monitor changes in host gene expression during disease development. This analysis was performed at 4 d postinoculation, when bacteria were actively multiplying and no wilt symptoms were yet visible; and at 8 d postinoculation, when bacterial growth approached saturation and typical wilt symptoms were observed. Of the 9,254 tomato genes represented on the array, 122 were differentially expressed in Cmminfected plants, compared with mock-inoculated plants. Functional classification of Cmm-responsive genes revealed that Cmm activated typical basal defense responses in the host, including induction of defense-related genes, production and scavenging of free oxygen radicals, enhanced protein turnover, and hormone synthesis. Cmm infection also induced a subset of host genes involved in ethylene biosynthesis and response. After inoculation with Cmm, Never ripe (Nr) mutant plants, impaired in ethylene perception, and transgenic plants with reduced ethylene synthesis showed significant delay in the appearance of wilt symptoms, compared with wild-type plants. The retarded wilting in Nr plants was a specific effect of ethylene insensitivity, and was not due to altered expression of defense-related genes, reduced bacterial populations, or decreased ethylene synthesis. Taken together, our results indicate that host-derived ethylene plays an important role in regulation of the tomato susceptible response to Cmm. © 2008 American Society of Plant Biologists.
Scientific Publication
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