אסיף מאגר המחקר החקלאי

Linkage map of Cucumis melo including phenotypic traits and sequence-characterized genes

Year:

2003

Source of publication :

Authors :

Volume :

46

Co-Authors:

Silberstein, L., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Kovalski, I., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Brotman, Y., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Perin, C., INRA, Stn. Genet. d'Amelioration Fruit L., B.P. 94, 84143 Montfavet Cedex, France
Dogimont, C., INRA, Stn. Genet. d'Amelioration Fruit L., B.P. 94, 84143 Montfavet Cedex, France
Pitrat, M., INRA, Stn. Genet. d'Amelioration Fruit L., B.P. 94, 84143 Montfavet Cedex, France
Klingler, J., CSIRO Entomology, Private Bag 5, Wembley, WA 6913, Australia
Thompson, G., Department of Applied Science, University of Arkansas, Little Rock, AR 72204, United States
Portnoy, V., Agricultural Research Organization, Newe Yaar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Katzir, N., Agricultural Research Organization, Newe Yaar Research Center, P.O. Box 1021, Ramat-Yishay 30095, Israel
Perl-Treves, R., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel

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Abstract:

A new linkage map of Cucumis melo, derived from the F2 progeny of a cross between PI 414723 and C. melo 'TopMark' is presented. The map spans a total of 1421 cM and includes 179 points consisting of random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), inter-simple sequence repeats (ISSRs), simple sequence repeats (SSRs), and restriction fragment length polymorphism (RFLP) markers. The map also includes an aphid resistance trait (Vat) and the sex type gene, andromonoecious (a), the two of which are important in resistance breeding and the control of hybrid seed production, as well as a seed-color gene, Wt-2. Most RFLPs represent sequence-characterized cDNA probes from C. melo and Cucumis sativus. These include resistance gene homologues and genes involved in various aspects of plant development and metabolism. A sub-set of our SSR and RFLP markers were also mapped, as part of this study, on additional mapping populations that were published for this species. This provides important reference points ("anchors"), enabling us to identify several linkage groups with respect to other melon maps.

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