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פותח על ידי קלירמאש פתרונות בע"מ -
Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?
Year:
2000
Source of publication :
Cryo-Letters
Authors :
פרל, מיכל
;
.
Volume :
21
Co-Authors:
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearl, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
179
To page:
186
(
Total pages:
8
)
Abstract:
Ram, fowl and bee spermatozoa, and oocytes of cows and zebrafish were used to study lipid membrane profiles, chilling sensitivity and lipid-phase transitions. The integrity of the membranes was determined by carboxyfluorescein diacetate (cFDA) staining following exposure for 15 minutes to low-temperatures. Ram and fowl spermatozoa showed differing degrees of loss of membrane integrity. Surprisingly, bee spermatozoa did not show any sensitivity to chilling, and their membranes remained intact down to 0°C. In bovine oocytes (at the GV stage) chilling injury was very severe at 16°C (membrane integrity decreased by 50%). Lipid phase transition (LPT) and membrane fluidity, which were evaluated by Fourier transform infrared (FTIR) microscopy, and fluorescence polarization, showed phase transitions at the same temperatures as caused damage (between 30 and 12°C). The membrane lipid profiles showed high concentrations of polyunsaturated fatty acids (PUFA) in cold-sensitive ram spermatozoa and zebrafish oocytes, but the ratio between PUFA and saturated fatty acids was highest in cold-resistant bee spermatozoa and lowest in cold-sensitive bovine oocytes. These results suggest a close relationship among cold susceptibility, lipid phase transition and lipids profile in animal gametes.
Note:
Related Files :
chilling injury
cryopreservation
Gametes
Membranes
Oocytes
Spermatozoa
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23839
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:02
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Scientific Publication
Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?
21
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearl, M., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Zeron, Y., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Does lipid profile explain chilling sensitivity and membrane lipid phase transition of spermatozoa and oocytes?
Ram, fowl and bee spermatozoa, and oocytes of cows and zebrafish were used to study lipid membrane profiles, chilling sensitivity and lipid-phase transitions. The integrity of the membranes was determined by carboxyfluorescein diacetate (cFDA) staining following exposure for 15 minutes to low-temperatures. Ram and fowl spermatozoa showed differing degrees of loss of membrane integrity. Surprisingly, bee spermatozoa did not show any sensitivity to chilling, and their membranes remained intact down to 0°C. In bovine oocytes (at the GV stage) chilling injury was very severe at 16°C (membrane integrity decreased by 50%). Lipid phase transition (LPT) and membrane fluidity, which were evaluated by Fourier transform infrared (FTIR) microscopy, and fluorescence polarization, showed phase transitions at the same temperatures as caused damage (between 30 and 12°C). The membrane lipid profiles showed high concentrations of polyunsaturated fatty acids (PUFA) in cold-sensitive ram spermatozoa and zebrafish oocytes, but the ratio between PUFA and saturated fatty acids was highest in cold-resistant bee spermatozoa and lowest in cold-sensitive bovine oocytes. These results suggest a close relationship among cold susceptibility, lipid phase transition and lipids profile in animal gametes.
Scientific Publication
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