אסיף מאגר המחקר החקלאי

Species identification of soil-borne Orobanche seeds by DNA fingerprinting

Year:

1997

Source of publication :

Authors :

Volume :

58

Co-Authors:

Portnoy, V.H., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Katzir, N., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Joel, D.M., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel

Facilitators :

Link :

Abstract:

DNA was extracted from single soil-borne seeds of Orobanche ramosa L., O. crenata Forsk, O. cumana Loeffl, and O. aegyptiaca. Species identification was then possible by RAPD-PCR, based on specific DNA markers, and could be verified by Southern hybridization with specific probes. Seed identification was also possible for dead (tetrazolium-negative) seeds. This method is a powerful diagnostic tool for Orobanche classification in agricultural fields.

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