Co-Authors:
Aly, R., Department of Weed Research, A.R.O., Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Granot, D., Dept. Field Crops and Nat. Resources, A.R.O., Volcani Center, Bet Dagan, Israel
Mahler-Slasky, Y., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Halpern, N., Department of Weed Research, A.R.O., Newe Ya'ar Research Center, P.O. Box 1021, Ramat Yishay 30095, Israel
Nir, D., Dept. Field Crops and Nat. Resources, A.R.O., Volcani Center, Bet Dagan, Israel
Galun, E., Department of Plant Genetics, Weizmann Institute of Science, Rehovot, Israel
Abstract:
Sarcotoxin IA is a cecropin-type antibacterial protein produced by the flesh fly, Sarcophaga peregrina. Similar to other bactericidal small proteins produced by insects, sarcotoxin IA is released into the hemolymph of larvae and nymphs upon mechanical injury or bacterial infection. The gene (sarco) that encodes this toxin was introduced into Saccharomyces cerevisiae yeast cells and was expressed under a constitutive yeast promoter. The transformed yeast cells were grown in a liquid medium, and a peptide with a similar molecular size to that of the mature sarcotoxin IA was detected in the medium by Western blot analysis. The secreted sarcotoxin-like peptide (SLP) had a potent cytotoxic effect against several bacteria, including plant pathogenic bacteria, similar to the toxic effects of the authentic sarcotoxin IA. Erwinia carotovora was more susceptible to the toxic medium than Pseudomonas solanacearum and Pseudomonas syringae pv. lachrymans. Thus, yeast may be used in the production of such proteins for employment against various bacterial pathogens.
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