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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of bacterial chitinase protein in tobacco leaves using two photosynthetic gene promoters
Year:
1988
Source of publication :
MGG Molecular & General Genetics
Authors :
גדעוני, דוד
;
.
Volume :
212
Co-Authors:
Jones, J.D.G., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Dean, C., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Gidoni, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Gilbert, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Bond-Nutter, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Lee, R., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Bedbrook, J., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Dunsmuir, P., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Facilitators :
From page:
536
To page:
542
(
Total pages:
7
)
Abstract:
A bacterial chitinase gene from Serratia marcescens (chiA) was fused to (i) a promoter of the ribulose bisphosphate carboxylase small subunit (rbcS) gene and (ii) two different chlorophyll a/b binding protein (cab) gene promoters from petunia. The resulting constructions were introduced into Agrobacterium Ti plasmid-based plant cell transformation vectors and used to generate multiple independent transgenic tobacco plants. ChiA mRNA and protein levels were measured in these plants. On average, the rbcS/chiA fusion gave rise to threefold more chiA mRNA than either cab/chiA fusion. We investigated the influence of sequences around the translational initiation ATG codon on the level of ChiA protein. The rbcS/chiA and cab/chiA fusions in which the sequence in the vicinity of the translational initiation codon is ACC ATGGC gave rise to transformants with higher levels of ChiA protein than those carrying a cab/chiA fusion with the sequence CAT ATGCG in the same region. This difference in translational efficiency is consistent with previous findings on preferred sequences in this region of the mRNA. In those transformants showing the highest level of ChiA expression, ChiA protein accumulated to about 0.25% of total soluble leaf protein. These plants contained significantly higher chitinase enzymatic activity than control plants. © 1988 Springer-Verlag.
Note:
Related Files :
Agrobacterium
Chitinase activity
Chlorophyll a/b binding protein
Promoter comparisons
Small subunit of ribulose bisphosphate carboxylase
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00330861
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23858
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:03
Scientific Publication
Expression of bacterial chitinase protein in tobacco leaves using two photosynthetic gene promoters
212
Jones, J.D.G., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Dean, C., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Gidoni, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Gilbert, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Bond-Nutter, D., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Lee, R., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Bedbrook, J., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Dunsmuir, P., Advanced Genetic Sciences Inc., 6701 San Pablo Ave., Oakland, 94608, CA, United States
Expression of bacterial chitinase protein in tobacco leaves using two photosynthetic gene promoters
A bacterial chitinase gene from Serratia marcescens (chiA) was fused to (i) a promoter of the ribulose bisphosphate carboxylase small subunit (rbcS) gene and (ii) two different chlorophyll a/b binding protein (cab) gene promoters from petunia. The resulting constructions were introduced into Agrobacterium Ti plasmid-based plant cell transformation vectors and used to generate multiple independent transgenic tobacco plants. ChiA mRNA and protein levels were measured in these plants. On average, the rbcS/chiA fusion gave rise to threefold more chiA mRNA than either cab/chiA fusion. We investigated the influence of sequences around the translational initiation ATG codon on the level of ChiA protein. The rbcS/chiA and cab/chiA fusions in which the sequence in the vicinity of the translational initiation codon is ACC ATGGC gave rise to transformants with higher levels of ChiA protein than those carrying a cab/chiA fusion with the sequence CAT ATGCG in the same region. This difference in translational efficiency is consistent with previous findings on preferred sequences in this region of the mRNA. In those transformants showing the highest level of ChiA expression, ChiA protein accumulated to about 0.25% of total soluble leaf protein. These plants contained significantly higher chitinase enzymatic activity than control plants. © 1988 Springer-Verlag.
Scientific Publication
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