אסיף מאגר המחקר החקלאי

Site-specific recombination of asymmetric lox sites mediated by a heterotetrameric Cre recombinase complex

Year:

2006

Source of publication :

Authors :

Volume :

14

Co-Authors:

Santoro, S.W., Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, United States
Schultz, P.G., Department of Chemistry and the Skaggs Institute for Chemical Biology, Scripps Research Institute, SR202, 10550 North Torrey Pines Road, San Diego, CA 92037, United States
 

Facilitators :

Link :

Abstract:

Previous reports have demonstrated that new Cre recombinase specificities can be developed for symmetrically designed lox mutants through directed evolution. The development of Cre variants that allow the recombination of true asymmetric lox mutant sites has not yet been addressed, however. In the present study, we demonstrate that a mixture of two different site-specific Cre recombinase molecules (wt Cre and a mutant Cre) catalyzes efficient recombination between two asymmetric lox sites in vitro, presumably via formation of a functionally active heterotetrameric complex. The results may broaden the application of site-specific recombination in basic and applied research, including the custom-design of recombinases for natural, asymmetric, and lox-related target sequences present in the genome. Future applications may potentially include genomic manipulations, for example, site-specific integrations, deletions or substitutions within precise regions of the genomes of mammalians and other organisms. © 2005 Elsevier Ltd. All rights reserved.

Note:

Related Files :

תוכן קשור

More details

You may also be interested in