Co-Authors:
Lewinsohn, E., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, IL-76100, Israel
Berman, E., Department of Organic Chemistry, The Wweizman Institute of Science, Rehovot, IL-76100, Israel
Mazur, Y., Department of Organic Chemistry, The Wweizman Institute of Science, Rehovot, IL-76100, Israel
Gressel, J., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, IL-76100, Israel
Abstract:
The ability of citrus cultures to biotransform flavanones was tested. Only one undifferentiated sour orange (Citrus aurantium L.) ovule derived cell culture glucosylated exogenous naringenin at position 7 and further rhamnosylated the product at position 6 of the glucose, synthesizing narirutin. The products were verified by 1H-NMR. Other ovule-derived cell lines, one from sour orange (Citrus aurantium L.) and one from a Poorman × Poncirus trifoliata (L.) Raf. hybrid were only able to glucosylate exogenous naringenin and hesperetin at position 7, as previously shown in C. paradisi Macf. cv. Duncan cell cultures. TLC analyses indicated that cultures of 'Ponderosa' lemon (C. limon (L.) Burm. f. cv. Ponderosa) and grapefruit (C. paradisi Macf. cv. White Marsh) also glucosylated exogenous hesperetin at position 7. None of the cell-lines tested accumulated flavanone-glycosides without exogenously supplying aglycones. © 1989.