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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Effect of substitution of the amino termini of coat proteins of distinct potyvirus species on viral infectivity and host specificity
Year:
2003
Authors :
גל-און, עמית
;
.
רקח, בנימין
;
.
Volume :
63
Co-Authors:
Ullah, Z., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States, Dept. of Biochem. and Molec. Biology, Michigan State University, East Lansing, MI 48824, United States
Chai, B., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States, Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, United States
Hammar, S., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States
Raccah, B., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Grumet, R., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States
Facilitators :
From page:
129
To page:
139
(
Total pages:
11
)
Abstract:
The amino terminus (NT) of the potyvirus coat protein (CP), which is necessary for aphid transmission and systemic infection, is externally located on the virion, strongly antigenic, and highly variable in length and sequence. Chimeras of Zucchini yellow mosaic virus (ZYMV) were constructed by substitution of the native CP-NT with CP-NTs of potyviruses with overlapping (Watermelon mosaic virus, WMV) and non-overlapping (Tobacco etch virus, TEV) host range. The chimeric viruses produced strong initial symptoms on ZYMV-susceptible cucurbits. Approximately 6 weeks post-inoculation, zucchini and cucumber plants infected with the TEV chimera, exhibited a distinct recovery characterized by a loss of symptoms on young leaves, reduced virus titer, and virus-specific protection against secondary infection. The chimeric viruses did not overcome naturally occurring ZYMV resistance in cucumber, ZYMV-CP-mediated resistance in transgenic melons, or expand host range to TEV- or WMV-susceptible species. These results demonstrate that despite substantial variability in length and sequence, the CP-NTs from heterologous potyviruses facilitated systemic infection in ZYMV-susceptible cucurbits, but were not sufficient to cause infection in non-ZYMV hosts. The observed recovery response with the non-cucurbit CP-NT suggests that the CP-NT is important for biological efficiency of the virus and/or adaptation of the virus to its host. © 2003 Elsevier Ltd. All rights reserved.
Note:
Related Files :
Capsid protein
Cucumis melo
Cucumis sativus
Cucurbita
Cucurbita pepo
Cucurbita pepo L
Zucchini yellow mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.pmpp.2003.11.001
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23922
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:03
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Scientific Publication
Effect of substitution of the amino termini of coat proteins of distinct potyvirus species on viral infectivity and host specificity
63
Ullah, Z., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States, Dept. of Biochem. and Molec. Biology, Michigan State University, East Lansing, MI 48824, United States
Chai, B., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States, Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, United States
Hammar, S., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States
Raccah, B., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50-250, Israel
Grumet, R., Plant Breeding and Genetics Program, Michigan State University, East Lansing, MI 48824, United States
Effect of substitution of the amino termini of coat proteins of distinct potyvirus species on viral infectivity and host specificity
The amino terminus (NT) of the potyvirus coat protein (CP), which is necessary for aphid transmission and systemic infection, is externally located on the virion, strongly antigenic, and highly variable in length and sequence. Chimeras of Zucchini yellow mosaic virus (ZYMV) were constructed by substitution of the native CP-NT with CP-NTs of potyviruses with overlapping (Watermelon mosaic virus, WMV) and non-overlapping (Tobacco etch virus, TEV) host range. The chimeric viruses produced strong initial symptoms on ZYMV-susceptible cucurbits. Approximately 6 weeks post-inoculation, zucchini and cucumber plants infected with the TEV chimera, exhibited a distinct recovery characterized by a loss of symptoms on young leaves, reduced virus titer, and virus-specific protection against secondary infection. The chimeric viruses did not overcome naturally occurring ZYMV resistance in cucumber, ZYMV-CP-mediated resistance in transgenic melons, or expand host range to TEV- or WMV-susceptible species. These results demonstrate that despite substantial variability in length and sequence, the CP-NTs from heterologous potyviruses facilitated systemic infection in ZYMV-susceptible cucurbits, but were not sufficient to cause infection in non-ZYMV hosts. The observed recovery response with the non-cucurbit CP-NT suggests that the CP-NT is important for biological efficiency of the virus and/or adaptation of the virus to its host. © 2003 Elsevier Ltd. All rights reserved.
Scientific Publication
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