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פותח על ידי קלירמאש פתרונות בע"מ -
The estimation of cell death in suspension cultures evoked by phytotoxic compounds: Differences among techniques
Year:
1978
Source of publication :
Plant Science Letters
Authors :
זילכה, שמואל
;
.
Volume :
12
Co-Authors:
Zilkah, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
305
To page:
315
(
Total pages:
11
)
Abstract:
We have presented data evaluating and comparing three rapid methods of estimating phytotoxicity (precursor incorporation into macromolecules, membrane permeability to fluorescein and reduction of triphenyltetrazolium chloride) with three slower methods of estimating growth (packed volume, dry wt. and cell number). We have described the advantages and drawbacks of each technique, as well as cases of a specific sensitivity of a particular technique to some toxic compounds. Despite all the differences, there was an overall correlation among all the techniques and the choice of technique depends on a desire to assay for a particular type of toxicity. As with other biological systems, these data demonstrate the problems in establishing both the time as well as the occurrence of death. © 1978.
Note:
Related Files :
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תוכן קשור
More details
DOI :
10.1016/0304-4211(78)90083-4
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23964
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
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Scientific Publication
The estimation of cell death in suspension cultures evoked by phytotoxic compounds: Differences among techniques
12
Zilkah, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
Gressel, J., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, Israel
The estimation of cell death in suspension cultures evoked by phytotoxic compounds: Differences among techniques
We have presented data evaluating and comparing three rapid methods of estimating phytotoxicity (precursor incorporation into macromolecules, membrane permeability to fluorescein and reduction of triphenyltetrazolium chloride) with three slower methods of estimating growth (packed volume, dry wt. and cell number). We have described the advantages and drawbacks of each technique, as well as cases of a specific sensitivity of a particular technique to some toxic compounds. Despite all the differences, there was an overall correlation among all the techniques and the choice of technique depends on a desire to assay for a particular type of toxicity. As with other biological systems, these data demonstrate the problems in establishing both the time as well as the occurrence of death. © 1978.
Scientific Publication
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