נגישות
menu      
חיפוש מתקדם
Journal of Environmental Quality
Masaphy, S., Migal Technological Center, Kiryat Shmona, P.O.B. 90000, Rosh Pinna 12100, Israel
Fahima, T., Inst. of Evolution, Univ. of Haifa, Israel
Levanon, D., Inst. of Soils and Water, Volcani Center, Bet Dagan, Israel
Henis, Y., Faculty of Agric., Hebrew Univ. of Jerusalem, Rehovot, Israel
Mingelgrin, U., Inst. of Soils and Water, Volcani Center, Bet Dagan, Israel
The degradation of parathion by a Xanthomonas sp. and by its crude enzyme extract in the presence of Na-montmorillonite and albumin, were studied to determine the influence of both organic and inorganic macrospecies on the availability of parathion (O,O diethyl-O-p-nitrophenyl phosphorothioate) for biological degradation in soil and water. Parathion hydrolysis, both by the bacterium and by its crude enzyme extract, was retarded by the clay. Parathion was rapidly adsorbed by the clay, reaching equilibrium within 6 h at a Kd value of 76 L/kg, but parathion adsorption was considerably higher on the protein-loaded clay. Enzyme inactivation, due to its adsorption by the clay, was the dominant cause for the decrease in parathion degradation in the presence of clay, while parathion adsorption had a smaller effect. The parathion desorption rate was very high; therefore, sorbed parathion was readily available for bacterial and enzymatic hydrolysis in solution. Addition of albumin to the crude enzyme extract stabilized the enzymatic activity in clay-free solution, but did not prevent the inactivation of parathion hydrolase by the clay.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Parathion degradation by Xanthomonas sp. and its crude enzyme extract in clay suspensions
25
Masaphy, S., Migal Technological Center, Kiryat Shmona, P.O.B. 90000, Rosh Pinna 12100, Israel
Fahima, T., Inst. of Evolution, Univ. of Haifa, Israel
Levanon, D., Inst. of Soils and Water, Volcani Center, Bet Dagan, Israel
Henis, Y., Faculty of Agric., Hebrew Univ. of Jerusalem, Rehovot, Israel
Mingelgrin, U., Inst. of Soils and Water, Volcani Center, Bet Dagan, Israel
Parathion degradation by Xanthomonas sp. and its crude enzyme extract in clay suspensions
The degradation of parathion by a Xanthomonas sp. and by its crude enzyme extract in the presence of Na-montmorillonite and albumin, were studied to determine the influence of both organic and inorganic macrospecies on the availability of parathion (O,O diethyl-O-p-nitrophenyl phosphorothioate) for biological degradation in soil and water. Parathion hydrolysis, both by the bacterium and by its crude enzyme extract, was retarded by the clay. Parathion was rapidly adsorbed by the clay, reaching equilibrium within 6 h at a Kd value of 76 L/kg, but parathion adsorption was considerably higher on the protein-loaded clay. Enzyme inactivation, due to its adsorption by the clay, was the dominant cause for the decrease in parathion degradation in the presence of clay, while parathion adsorption had a smaller effect. The parathion desorption rate was very high; therefore, sorbed parathion was readily available for bacterial and enzymatic hydrolysis in solution. Addition of albumin to the crude enzyme extract stabilized the enzymatic activity in clay-free solution, but did not prevent the inactivation of parathion hydrolase by the clay.
Scientific Publication
You may also be interested in