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Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii. I. Characterization of attachment to Botrytis cinerea
Year:
1991
Authors :
דרובי, סמיר
;
.
חלוץ, עדו
;
.
Volume :
39
Co-Authors:
Wisniewski, M., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Biles, C., New Mexico State University, Department of Entomology, Plant Pathology and Weed Science, Las Cruces, NM, United States
Droby, S., Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
McLaughlin, R., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Wilson, C., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Chalutz, E., Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
245
To page:
258
(
Total pages:
14
)
Abstract:
An isolate (87) of the yeast Pichia guilliermondii, protects apples from postharvest fruit rotting fungi Botrytis cinerea and Penicillium expansum. In order to examine the yeast-pathogen interaction, B. cinerea was grown on agar plates overlayed with cellophane. Effective and non-effective yeast isolates were applied near the young hyphal growth. Samples were taken 24 h later from the area where the fungi and yeast had intersected. Light microscopy revealed a general attachment of the effective biocontrol agent P. guilliermondii (isolate 87) and a non-effective isolate (117) of Debaryomyces hansenii. Low temperature scanning electron microscopy (LTSEM) indicated that both species of yeast attached to the fungal hyphae, but the 87 isolate attached fastidiously. Twenty-four hours after applying the 87 isolate to B. cinerea, pitting and collapse of the hyphae were observed. These observations were confirmed using transmission electron microscopy. These features were not observed with the ineffective isolate of D. hansenii. Further experiments indicated that attachment of P. guilliermondii to hyphae of B. cinerea could be blocked by agents that alter protein integrity (salts, proteases, etc.) and certain sugars. Isolates of both species produced β-(1-3) glucanase when cultured in various carbon sources and on cell walls of fruit rotting pathogens. Culture supernatants from P. guilliermondii, however, yielded two- to five-fold more β-(1-3) glucanase activity compared with D. hansenii. Data indicate that tenacious attachment, along with secretion of cell wall degrading enzymes, may play a role in the biocontrol activity of this yeast antagonist. © 1991.
Note:
Related Files :
Botrytis cinerea
food storage
Mode of action
Pichia guilliermondii
Postharvest biocontrol
postharvest treatment
technology and storage
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0885-5765(91)90033-E
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
23978
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii. I. Characterization of attachment to Botrytis cinerea
39
Wisniewski, M., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Biles, C., New Mexico State University, Department of Entomology, Plant Pathology and Weed Science, Las Cruces, NM, United States
Droby, S., Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
McLaughlin, R., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Wilson, C., United States Department of Agriculture, Agricultural Research Service, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, United States
Chalutz, E., Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Mode of action of the postharvest biocontrol yeast, Pichia guilliermondii. I. Characterization of attachment to Botrytis cinerea
An isolate (87) of the yeast Pichia guilliermondii, protects apples from postharvest fruit rotting fungi Botrytis cinerea and Penicillium expansum. In order to examine the yeast-pathogen interaction, B. cinerea was grown on agar plates overlayed with cellophane. Effective and non-effective yeast isolates were applied near the young hyphal growth. Samples were taken 24 h later from the area where the fungi and yeast had intersected. Light microscopy revealed a general attachment of the effective biocontrol agent P. guilliermondii (isolate 87) and a non-effective isolate (117) of Debaryomyces hansenii. Low temperature scanning electron microscopy (LTSEM) indicated that both species of yeast attached to the fungal hyphae, but the 87 isolate attached fastidiously. Twenty-four hours after applying the 87 isolate to B. cinerea, pitting and collapse of the hyphae were observed. These observations were confirmed using transmission electron microscopy. These features were not observed with the ineffective isolate of D. hansenii. Further experiments indicated that attachment of P. guilliermondii to hyphae of B. cinerea could be blocked by agents that alter protein integrity (salts, proteases, etc.) and certain sugars. Isolates of both species produced β-(1-3) glucanase when cultured in various carbon sources and on cell walls of fruit rotting pathogens. Culture supernatants from P. guilliermondii, however, yielded two- to five-fold more β-(1-3) glucanase activity compared with D. hansenii. Data indicate that tenacious attachment, along with secretion of cell wall degrading enzymes, may play a role in the biocontrol activity of this yeast antagonist. © 1991.
Scientific Publication
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