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פותח על ידי קלירמאש פתרונות בע"מ -
Conformation polymorphism of RNA transcripts derived from various PNRSV isolates is affected by the temperature of electrophoresis
Year:
1999
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
שפיגל, שרה
;
.
Volume :
105
Co-Authors:
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Facilitators :
From page:
413
To page:
415
(
Total pages:
3
)
Abstract:
Polymorphism of heterologous duplexes of RNA transcripts of prunus necrotic ringspot virus isolates was studied at different temperatures of electrophoresis. Duplexes made from complementary RNA transcripts originating from four virus isolates differed in their electrophoretic mobilities. The differential mobilities of double-stranded RNA transcripts increased with increasing temperature of electrophoresis. The mobility of single-stranded RNA, on the other hand, though affected by temperature during electrophoresis, was not simply correlated with sequence divergence. This finding further supports the hypothesis that transcript conformation is the basis for the differential mobility phenomenon.
Note:
Related Files :
Electrophoresis
genetic polymorphism
Ilarviruses
protein conformation
RNA
Strain differentiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1023/A:1008700120992
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24003
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
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Scientific Publication
Conformation polymorphism of RNA transcripts derived from various PNRSV isolates is affected by the temperature of electrophoresis
105
Rosner, A., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Maslenin, L., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Spiegel, S., Department of Virology, Volcani Center, ARO, Bet Dagan 50250, Israel
Conformation polymorphism of RNA transcripts derived from various PNRSV isolates is affected by the temperature of electrophoresis
Polymorphism of heterologous duplexes of RNA transcripts of prunus necrotic ringspot virus isolates was studied at different temperatures of electrophoresis. Duplexes made from complementary RNA transcripts originating from four virus isolates differed in their electrophoretic mobilities. The differential mobilities of double-stranded RNA transcripts increased with increasing temperature of electrophoresis. The mobility of single-stranded RNA, on the other hand, though affected by temperature during electrophoresis, was not simply correlated with sequence divergence. This finding further supports the hypothesis that transcript conformation is the basis for the differential mobility phenomenon.
Scientific Publication
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