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פותח על ידי קלירמאש פתרונות בע"מ -
Production of the mouse whey acidic protein in transgenic pigs during lactation.
Year:
1991
Source of publication :
Journal of Animal Science
Authors :
שמאי, אבי
;
.
Volume :
69
Co-Authors:
Shamay, A., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Solinas, S., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Pursel, V.G., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
McKnight, R.A., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Alexander, L., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Beattie, C., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Hennighausen, L., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Wall, R.J., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Facilitators :
From page:
4552
To page:
4562
(
Total pages:
11
)
Abstract:
The mouse whey acidic protein (WAP) gene was introduced into the genome of pigs and its expression was analyzed in the mammary gland. Mouse WAP was detected in milk of lactating females from five lines at levels between .5 and 1.5 g/liter, thereby representing as much as 2% of the total milk proteins. The corresponding mRNA was expressed in mammary tissue at levels similar to those of pig beta-lactoglobulin and beta-casein. The pattern of WAP secretion in three pigs over a period of 6 wk was quantitatively similar to that of pig beta-lactoglobulin. From the eight transgenic pigs analyzed, three successfully completed one lactational period, but five pigs stopped lactating a few days after parturition. Our results show that it is possible to produce large quantities of a foreign protein in milk of pigs over a full lactational period. However, expression of WAP can compromise the mammary gland and render it nonfunctional.
Note:
Related Files :
DNA
Female
Genetics
lactation
Male
mice
milk
RNA
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24022
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Production of the mouse whey acidic protein in transgenic pigs during lactation.
69
Shamay, A., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Solinas, S., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Pursel, V.G., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
McKnight, R.A., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Alexander, L., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Beattie, C., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Hennighausen, L., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Wall, R.J., Lab. of Biochem. and Metab., NIDDK, NIH, Bethesda, MD.
Production of the mouse whey acidic protein in transgenic pigs during lactation.
The mouse whey acidic protein (WAP) gene was introduced into the genome of pigs and its expression was analyzed in the mammary gland. Mouse WAP was detected in milk of lactating females from five lines at levels between .5 and 1.5 g/liter, thereby representing as much as 2% of the total milk proteins. The corresponding mRNA was expressed in mammary tissue at levels similar to those of pig beta-lactoglobulin and beta-casein. The pattern of WAP secretion in three pigs over a period of 6 wk was quantitatively similar to that of pig beta-lactoglobulin. From the eight transgenic pigs analyzed, three successfully completed one lactational period, but five pigs stopped lactating a few days after parturition. Our results show that it is possible to produce large quantities of a foreign protein in milk of pigs over a full lactational period. However, expression of WAP can compromise the mammary gland and render it nonfunctional.
Scientific Publication
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