נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon
Year:
2005
Source of publication :
Theoretical and Applied Genetics
Authors :
פורטנוי, ויטלי
;
.
קציר, נורית
;
.
Volume :
110
Co-Authors:
Brotman, Y., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Kovalski, I., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Dogimont, C., INRA, Centre de Recherche d'Avignon, U. Genet. d'Amelioration Fruits L., BP 94, 84143 Montfavet Cedex, France
Pitrat, M., INRA, Centre de Recherche d'Avignon, U. Genet. d'Amelioration Fruits L., BP 94, 84143 Montfavet Cedex, France
Portnoy, V., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay, Israel
Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay, Israel
Perl-Treves, R., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Facilitators :
From page:
337
To page:
345
(
Total pages:
9
)
Abstract:
In melon, the Fom-1 gene confers monogenic resistance against the soil-borne fungus Fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked Prv gene specifies resistance against the papaya ring spot virus. Markers linked to these resistance (R) genes were identified using two recombinant inbred line populations, derived from crosses between Cucumis melo Védrantais and C. melo PI 161375, and between C. melo Védrantais and C. melo PI 414723, respectively. Using bulked segregant analysis, as well as systematic scoring of the mapping populations, we developed two amplified fragment length polymorphism markers, two random amplified polymorphic DNA markers and five restriction fragment length polymorphism (RFLP) markers linked to this locus. Four of the RFLP sequences bear homology to nucleotide-binding site-leucine-rich repeat R genes, indicating the presence of a significant R-gene cluster in this locus. Our study provides the most closely linked markers published so far for these important traits. It also improves the resolution of the whole linkage group IX, which was difficult to order in our previous studies. Two of the markers were converted to cleaved amplified polymorphic sequence markers to facilitate their application in marker-assisted selection. Testing these two markers in several melon lines revealed different marker haplotypes in the melon germplasm and supported multiple, independent origin of the Fusarium races 0 and 2 resistance trait. © Springer-Verlag 2004.
Note:
Related Files :
Carica papaya
chromosome mapping
Cucumis melo
DNA
fungi
Fusarium
genetic markers
Genetics
Nucleic acids
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-004-1845-z
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24031
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
You may also be interested in
Scientific Publication
Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon
110
Brotman, Y., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Kovalski, I., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Dogimont, C., INRA, Centre de Recherche d'Avignon, U. Genet. d'Amelioration Fruits L., BP 94, 84143 Montfavet Cedex, France
Pitrat, M., INRA, Centre de Recherche d'Avignon, U. Genet. d'Amelioration Fruits L., BP 94, 84143 Montfavet Cedex, France
Portnoy, V., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay, Israel
Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, Ramat Yishay, Israel
Perl-Treves, R., Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 52900, Israel
Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon
In melon, the Fom-1 gene confers monogenic resistance against the soil-borne fungus Fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked Prv gene specifies resistance against the papaya ring spot virus. Markers linked to these resistance (R) genes were identified using two recombinant inbred line populations, derived from crosses between Cucumis melo Védrantais and C. melo PI 161375, and between C. melo Védrantais and C. melo PI 414723, respectively. Using bulked segregant analysis, as well as systematic scoring of the mapping populations, we developed two amplified fragment length polymorphism markers, two random amplified polymorphic DNA markers and five restriction fragment length polymorphism (RFLP) markers linked to this locus. Four of the RFLP sequences bear homology to nucleotide-binding site-leucine-rich repeat R genes, indicating the presence of a significant R-gene cluster in this locus. Our study provides the most closely linked markers published so far for these important traits. It also improves the resolution of the whole linkage group IX, which was difficult to order in our previous studies. Two of the markers were converted to cleaved amplified polymorphic sequence markers to facilitate their application in marker-assisted selection. Testing these two markers in several melon lines revealed different marker haplotypes in the melon germplasm and supported multiple, independent origin of the Fusarium races 0 and 2 resistance trait. © Springer-Verlag 2004.
Scientific Publication
You may also be interested in