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Plant Cell Reports
Watad, A.A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Kochba, M., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Nissim, A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Virology, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die. © 1995 Springer-Verlag.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
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תנאי שימוש
Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts
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Watad, A.A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Kochba, M., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Nissim, A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Virology, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die. © 1995 Springer-Verlag.
Scientific Publication
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