נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts
Year:
1995
Source of publication :
Plant Cell Reports
Authors :
גאבה, ויקטור
;
.
ותד, עבד
;
.
כוכבא, מרנינה
;
.
ניסים-לוי, עדה
;
.
Volume :
14
Co-Authors:
Watad, A.A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Kochba, M., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Nissim, A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Virology, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
345
To page:
348
(
Total pages:
4
)
Abstract:
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die. © 1995 Springer-Verlag.
Note:
Related Files :
Aconitum napellus
floating membrane raft
Liquid medium
solid medium micropropagation
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00238594
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24051
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts
14
Watad, A.A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Kochba, M., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Nissim, A., Department of Ornamental Horticulture, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Gaba, V., Department of Virology, ARO Volcani Center, P.O.B. 6, Bet Dagan, 50250, Israel
Improvement of Aconitum napellus micropropagation by liquid culture on floating membrane rafts
An efficient method was developed using floating membrane rafts (Liferaft™) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-supported rafts, supplemented by different levels of benzyl adenine (BA). Optimum shoot proliferation on solid medium required 4mg/l BA, whereas for expiants supported on rafts optimal proliferation was achieved at 0.25mg/l BA. Maximum shoot proliferation was found using the floating rafts (propagation ratio of 4.2 per month), 45% higher than the maximum value on solid medium. A similar value could be obtained on solid medium after a period of 2 months. The optimal response to BA was similar for fresh weight gain and shoot length. Growth in a shallow layer of liquid in shake flasks gives a similar shoot multiplication rate to that on floating rafts; however, submerged leaves brown and die. © 1995 Springer-Verlag.
Scientific Publication
You may also be interested in