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פותח על ידי קלירמאש פתרונות בע"מ -
Outreading promoters are located at both ends of the Gamma-delta transposon
Year:
1989
Source of publication :
MGG Molecular & General Genetics
Authors :
לרס, אמנון
;
.
Volume :
216
Co-Authors:
Lers, A., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Bitoun, R., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Zamir, A., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
138
To page:
143
(
Total pages:
6
)
Abstract:
Two plasmids were isolated containing oppositely oriented gamma-delta insertions between the wild-type transcription initiation site of the nifHDKY operon and the nifH coding sequence. The nifHDKY promoter of Klebsiella pneumoniae, similar to other nitrogen fixation (nif) promoters, normally requires the products of ntrA and nifA for activity. Mutations that allowed constitutive expression of the nifHDKY operon were searched for by transforming a plasmid, containing the regulatory region of this operon followed by an in-frame nifH'-'lacZ fusion, into a Lac-Escherichia coli strain (which contains no nifA) and screening for Lac+ derivatives. The plasmids described here were isolated from such derivatives and directed the constitutive expression of beta-galactosidase. Deletion analysis indicated that gamma-delta promoters other than those transcribing tnpA and tnpR were involved in this expression. Nuclease S1 mapping revealed outward-reading transcription initiation sites in both the gamma end and the delta end of the transposon. Most interestingly, one initiation site on each end was located in corresponding positions within the terminal inverted repeats. The sites were in the center of the longest sequence, of 12 bp, contiguously conserved between the terminal inverted repeats of gamma-delta and the related transposon Tn3. In gamma-delta and Tn3, this sequence has been recently implicated in transposase binding. These results suggest a possible interrelationship between transcription from the "end" promoters and transposition. © 1989 Springer-Verlag.
Note:
Related Files :
chromosome deletion
genetic engineering
Heredity
mutation
Tn1000
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/BF00332242
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24057
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Outreading promoters are located at both ends of the Gamma-delta transposon
216
Lers, A., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Bitoun, R., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Zamir, A., Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel
Outreading promoters are located at both ends of the Gamma-delta transposon
Two plasmids were isolated containing oppositely oriented gamma-delta insertions between the wild-type transcription initiation site of the nifHDKY operon and the nifH coding sequence. The nifHDKY promoter of Klebsiella pneumoniae, similar to other nitrogen fixation (nif) promoters, normally requires the products of ntrA and nifA for activity. Mutations that allowed constitutive expression of the nifHDKY operon were searched for by transforming a plasmid, containing the regulatory region of this operon followed by an in-frame nifH'-'lacZ fusion, into a Lac-Escherichia coli strain (which contains no nifA) and screening for Lac+ derivatives. The plasmids described here were isolated from such derivatives and directed the constitutive expression of beta-galactosidase. Deletion analysis indicated that gamma-delta promoters other than those transcribing tnpA and tnpR were involved in this expression. Nuclease S1 mapping revealed outward-reading transcription initiation sites in both the gamma end and the delta end of the transposon. Most interestingly, one initiation site on each end was located in corresponding positions within the terminal inverted repeats. The sites were in the center of the longest sequence, of 12 bp, contiguously conserved between the terminal inverted repeats of gamma-delta and the related transposon Tn3. In gamma-delta and Tn3, this sequence has been recently implicated in transposase binding. These results suggest a possible interrelationship between transcription from the "end" promoters and transposition. © 1989 Springer-Verlag.
Scientific Publication
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