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פותח על ידי קלירמאש פתרונות בע"מ -
Genome scan for nonadditive heterotic trait loci reveals mainly underdominant effects in Saccharomyces cerevisiae
Year:
2016
Source of publication :
Genome
Authors :
כהן, י'
;
.
לוי, יעל
;
.
לייבה, אפרת
;
.
פרידמן, איל
;
.
Volume :
59
Co-Authors:
Laiba, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Glikaite, I., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Pasternak, Z., Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Fridman, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Facilitators :
From page:
231
To page:
242
(
Total pages:
12
)
Abstract:
The overdominant model of heterosis explains the superior phenotype of hybrids by synergistic allelic interaction within heterozygous loci. To map such genetic variation in yeast, we used a population doubling time dataset of Saccharomyces cerevisiae 16 × 16 diallel and searched for major contributing heterotic trait loci (HTL). Heterosis was observed for the majority of hybrids, as they surpassed their best parent growth rate. However, most of the local heterozygous loci identified by genome scan were surprisingly underdominant, i.e., reduced growth. We speculated that in these loci adverse effects on growth resulted from incompatible allelic interactions. To test this assumption, we eliminated these allelic interactions by creating hybrids with local hemizygosity for the underdominant HTLs, as well as for control random loci. Growth of hybrids was indeed elevated for most hemizygous to HTL genes but not for control genes, hence validating the results of our genome scan. Assessing the consequences of local heterozygosity by reciprocal hemizygosity and allele replacement assays revealed the influence of genetic background on the underdominant effects of HTLs. Overall, this genome-wide study on a multi-parental hybrid population provides a strong argument against single gene overdominance as a major contributor to heterosis, and favors the dominance complementation model. © 2016 Published by NRC Research Press.
Note:
Related Files :
fungal genome
Genetics
Genome-wide association
Heterozygote
inheritance
Inheritance Patterns
phenotype
עוד תגיות
תוכן קשור
More details
DOI :
10.1139/gen-2015-0127
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24077
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Genome scan for nonadditive heterotic trait loci reveals mainly underdominant effects in Saccharomyces cerevisiae
59
Laiba, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Glikaite, I., Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Levy, Y., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Pasternak, Z., Department of Plant Pathology and Microbiology, Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Fridman, E., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet-Dagan, Israel
Genome scan for nonadditive heterotic trait loci reveals mainly underdominant effects in Saccharomyces cerevisiae
The overdominant model of heterosis explains the superior phenotype of hybrids by synergistic allelic interaction within heterozygous loci. To map such genetic variation in yeast, we used a population doubling time dataset of Saccharomyces cerevisiae 16 × 16 diallel and searched for major contributing heterotic trait loci (HTL). Heterosis was observed for the majority of hybrids, as they surpassed their best parent growth rate. However, most of the local heterozygous loci identified by genome scan were surprisingly underdominant, i.e., reduced growth. We speculated that in these loci adverse effects on growth resulted from incompatible allelic interactions. To test this assumption, we eliminated these allelic interactions by creating hybrids with local hemizygosity for the underdominant HTLs, as well as for control random loci. Growth of hybrids was indeed elevated for most hemizygous to HTL genes but not for control genes, hence validating the results of our genome scan. Assessing the consequences of local heterozygosity by reciprocal hemizygosity and allele replacement assays revealed the influence of genetic background on the underdominant effects of HTLs. Overall, this genome-wide study on a multi-parental hybrid population provides a strong argument against single gene overdominance as a major contributor to heterosis, and favors the dominance complementation model. © 2016 Published by NRC Research Press.
Scientific Publication
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