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פותח על ידי קלירמאש פתרונות בע"מ -
Development of genetic markers for the white grouper (Epinephelus aeneus)
Year:
2014
Source of publication :
Aquaculture (source)
Authors :
חולתא, גדעון
;
.
רון, מיכה
;
.
שיראק, אנדרי
;
.
Volume :
420-421
Co-Authors:
Dor, L., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Gorshkov, S., National Center for Mariculture, Israel Oceanographic and Limnological Research (NCM), Eilat, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:
The white grouper (Epinephelus aeneus) has high commercial value, yet genomic tools for marker assisted domestication and breeding of the species are lacking. In this research, a total of 610 published sequences of microsatellites in Epinephelus species were found, from which 222 were suitable for primer design. Heterologous markers for E. aeneus were developed from microsatellite sequences of Epinephelus awoara, Epinephelus septemfasciatus, Epinephelus fuscoguttatus, Epinephelus itajara, Epinephelus guttatus, Epinephelus akaara and Epinephelus lanceolatus. The yield of heterologous polymorphic microsatellites by species of origin ranged from 42 to 83%. The lowest yield was found for E. septemfasciatus (P=0.001), which is the most phylogenetically distant from E. aeneus. A total of 140 loci were found polymorphic in the wild-type E. aeneus population (WT) and 137 loci in its captive broodstock. The average number of alleles was 6.5 in the WT population and 3.5 in the captive population (P=3 e-25). A set of eight microsatellites with 6 to 10 alleles per marker in the WT population was developed for parentage analysis of the offspring population. Two males and two females were found to contribute genetic material in various pairing combinations, whereas one pair was shown to be the parents of most of the progeny (69.2%), suggesting the reproduction mode of E. aeneus as polygamous. The parentity test is being used to form a segregating population for linkage mapping of all available genetic markers, thereby creating the first-generation white grouper linkage map. © 2013 Elsevier B.V.
Note:
Related Files :
breeding
Developmental Biology
DNA
Epinephelus aeneus
Epinephelus awoara
phylogenetics
Promicrops lanceolatus
White grouper
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.aquaculture.2013.02.023
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24080
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
Scientific Publication
Development of genetic markers for the white grouper (Epinephelus aeneus)
420-421
Dor, L., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Gorshkov, S., National Center for Mariculture, Israel Oceanographic and Limnological Research (NCM), Eilat, Israel
Ron, M., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Hulata, G., Institute of Animal Science, Agricultural Research Organization, Bet Dagan, Israel
Development of genetic markers for the white grouper (Epinephelus aeneus)
The white grouper (Epinephelus aeneus) has high commercial value, yet genomic tools for marker assisted domestication and breeding of the species are lacking. In this research, a total of 610 published sequences of microsatellites in Epinephelus species were found, from which 222 were suitable for primer design. Heterologous markers for E. aeneus were developed from microsatellite sequences of Epinephelus awoara, Epinephelus septemfasciatus, Epinephelus fuscoguttatus, Epinephelus itajara, Epinephelus guttatus, Epinephelus akaara and Epinephelus lanceolatus. The yield of heterologous polymorphic microsatellites by species of origin ranged from 42 to 83%. The lowest yield was found for E. septemfasciatus (P=0.001), which is the most phylogenetically distant from E. aeneus. A total of 140 loci were found polymorphic in the wild-type E. aeneus population (WT) and 137 loci in its captive broodstock. The average number of alleles was 6.5 in the WT population and 3.5 in the captive population (P=3 e-25). A set of eight microsatellites with 6 to 10 alleles per marker in the WT population was developed for parentage analysis of the offspring population. Two males and two females were found to contribute genetic material in various pairing combinations, whereas one pair was shown to be the parents of most of the progeny (69.2%), suggesting the reproduction mode of E. aeneus as polygamous. The parentity test is being used to form a segregating population for linkage mapping of all available genetic markers, thereby creating the first-generation white grouper linkage map. © 2013 Elsevier B.V.
Scientific Publication
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