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פותח על ידי קלירמאש פתרונות בע"מ -
Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan
Year:
2004
Source of publication :
Plant Disease
Authors :
שפיגל, שרה
;
.
Volume :
88
Co-Authors:
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50 250, Israel
Kovalenko, E.M., Inst. of Fruit Grow. and Viticulture, Almaty, 480 060, Kazakhstan
Varga, A., Centre for Plant Health, Canadian Food Inspection Agency, Sidney, BC V8L 1H3, Canada
James, D., Centre for Plant Health, Canadian Food Inspection Agency, Sidney, BC V8L 1H3, Canada
Facilitators :
From page:
973
To page:
979
(
Total pages:
7
)
Abstract:
Plum pox virus (PPV) was detected in wild apricot and cultivated plum maintained in a germ plasm collection in Kazakhstan. Both isolates were typed as D strain, with no evidence of recombination. The virus was detected by triple-antibody sandwich enzyme-linked immunosorbent assay (ELISA) utilizing the universal PPV-specific monoclonal antibody (MAb) 5B as the secondary antibody, and by reverse-transcription polymerase chain reaction (RT-PCR) assay using primers that amplified a 243-bp fragment in the C-terminus of the coat protein (CP) coding region. Immunocapture (IC) RT-PCR was used to detect PPV in nine wild apricot accessions, including eight ELISA-negative and one ELISA-positive. The plum and apricot isolates reacted positively in Western blot assay with the universal MAb 5B, and negatively with the strain-M-specific MAb-AL. Restriction fragment length polymorphism analysis applied to the amplified 243-bp fragment showed that restriction sites for AluI and RsaI were present in the plum and apricot samples. An amplified 836-bp cDNA fragment derived from the P3-6K 1 coding region of both isolates had restriction profiles typical for strain D. Nucleotide identities of 99 to 100% were observed for the 243-bp fragments of the Kazakhstan isolates when compared with the corresponding regions of strain D, and 94 to 95% identity with strain M. Nucleotide sequence analysis of the entire CP coding region of the plum and apricot isolates resulted in the identification of a unique deletion of six nucleotides (two deduced proline amino acid residues) in the N-terminal region in the plum isolate. This is the first deletion of this nature observed among PPV isolates. The DAG motif was present in both isolates. Several nucleotide substitutions in the CP coding region were common to the plum and apricot isolates and appear to be unique to the Kazakstan isolates. This suggests a close relationship between the isolates.
Note:
Related Files :
Amino Acids
Bioassay
enzymes
Nucleotide deletions
Plum Pox Virus
Plum pox virus (PPV)
Prunus armeniaca
Viruses
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24082
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:04
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Scientific Publication
Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan
88
Spiegel, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50 250, Israel
Kovalenko, E.M., Inst. of Fruit Grow. and Viticulture, Almaty, 480 060, Kazakhstan
Varga, A., Centre for Plant Health, Canadian Food Inspection Agency, Sidney, BC V8L 1H3, Canada
James, D., Centre for Plant Health, Canadian Food Inspection Agency, Sidney, BC V8L 1H3, Canada
Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan
Plum pox virus (PPV) was detected in wild apricot and cultivated plum maintained in a germ plasm collection in Kazakhstan. Both isolates were typed as D strain, with no evidence of recombination. The virus was detected by triple-antibody sandwich enzyme-linked immunosorbent assay (ELISA) utilizing the universal PPV-specific monoclonal antibody (MAb) 5B as the secondary antibody, and by reverse-transcription polymerase chain reaction (RT-PCR) assay using primers that amplified a 243-bp fragment in the C-terminus of the coat protein (CP) coding region. Immunocapture (IC) RT-PCR was used to detect PPV in nine wild apricot accessions, including eight ELISA-negative and one ELISA-positive. The plum and apricot isolates reacted positively in Western blot assay with the universal MAb 5B, and negatively with the strain-M-specific MAb-AL. Restriction fragment length polymorphism analysis applied to the amplified 243-bp fragment showed that restriction sites for AluI and RsaI were present in the plum and apricot samples. An amplified 836-bp cDNA fragment derived from the P3-6K 1 coding region of both isolates had restriction profiles typical for strain D. Nucleotide identities of 99 to 100% were observed for the 243-bp fragments of the Kazakhstan isolates when compared with the corresponding regions of strain D, and 94 to 95% identity with strain M. Nucleotide sequence analysis of the entire CP coding region of the plum and apricot isolates resulted in the identification of a unique deletion of six nucleotides (two deduced proline amino acid residues) in the N-terminal region in the plum isolate. This is the first deletion of this nature observed among PPV isolates. The DAG motif was present in both isolates. Several nucleotide substitutions in the CP coding region were common to the plum and apricot isolates and appear to be unique to the Kazakstan isolates. This suggests a close relationship between the isolates.
Scientific Publication
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