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חיפוש מתקדם
Tree Genetics and Genomes
Freiman, Z.E., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Doron-Faigenboim, A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Dasmohapatra, R., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Yablovitz, Z., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Flaishman, M.A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
The Ficus carica L. (Moraceae) tree belongs to one of the largest genera of angiosperms and bears a unique closed inflorescence structure. Ripe fresh figs are highly perishable and require delicate postharvest handling. Studying the pathways that lead to fig ripening may provide additional ways of extending their storage life. We selected four developmental stages of fig fruit for transcriptome sequencing and analysis to identify the major active metabolic pathways and transcription factors during fig fruit ripening. We found 12,751 unigenes, 93 % of which were homologous to at least one nonredundant database sequence, and 46,927 singletons, 39 % with a matching sequence from the nonredundant database. Differential activity related to photosynthesis, anthocyanin and volatile metabolism, cell wall and wax metabolism, cell expansion, transcription, DNA metabolism and organization was traced. In addition, ethylene-synthesis genes were identified. Finally, 516 unigenes encoding transcription factors were found which were active in the regulation of early and late ripening processes. Focusing on eight FcMADS-box transcription factors revealed three genes encoding members of the AGL2 (SEP) subfamily, which is closely associated with ripening regulation. This study provides expressed-gene dataset for multiple developmental stages of fig fruit (F. carica), and analysis directed to ripening metabolism, control, and regulation. It provides a potential platform for further studies of this unique plant family and contributes to ripening process research in nonmodel systems. © 2014 Springer-Verlag Berlin Heidelberg.
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הספר "אוצר וולקני"
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תנאי שימוש
High-throughput sequencing analysis of common fig (Ficus carica L.) transcriptome during fruit ripening
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Freiman, Z.E., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel
Doron-Faigenboim, A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Dasmohapatra, R., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Yablovitz, Z., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel
Flaishman, M.A., Institute of Plant Sciences, Agricultural Research Organization, P.O. Box 6, Bet-Dagan, 50250, Israel, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
High-throughput sequencing analysis of common fig (Ficus carica L.) transcriptome during fruit ripening
The Ficus carica L. (Moraceae) tree belongs to one of the largest genera of angiosperms and bears a unique closed inflorescence structure. Ripe fresh figs are highly perishable and require delicate postharvest handling. Studying the pathways that lead to fig ripening may provide additional ways of extending their storage life. We selected four developmental stages of fig fruit for transcriptome sequencing and analysis to identify the major active metabolic pathways and transcription factors during fig fruit ripening. We found 12,751 unigenes, 93 % of which were homologous to at least one nonredundant database sequence, and 46,927 singletons, 39 % with a matching sequence from the nonredundant database. Differential activity related to photosynthesis, anthocyanin and volatile metabolism, cell wall and wax metabolism, cell expansion, transcription, DNA metabolism and organization was traced. In addition, ethylene-synthesis genes were identified. Finally, 516 unigenes encoding transcription factors were found which were active in the regulation of early and late ripening processes. Focusing on eight FcMADS-box transcription factors revealed three genes encoding members of the AGL2 (SEP) subfamily, which is closely associated with ripening regulation. This study provides expressed-gene dataset for multiple developmental stages of fig fruit (F. carica), and analysis directed to ripening metabolism, control, and regulation. It provides a potential platform for further studies of this unique plant family and contributes to ripening process research in nonmodel systems. © 2014 Springer-Verlag Berlin Heidelberg.
Scientific Publication
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