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פותח על ידי קלירמאש פתרונות בע"מ -
The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia
Year:
2004
Source of publication :
Theoretical and Applied Genetics
Authors :
אורן-שמיר, מיכל
;
.
בורובסקי, ילנה
;
.
עובדיה, רינת
;
.
פארן, אילן
;
.
Volume :
109
Co-Authors:
Borovsky, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Oren-Shamir, M., Dept. of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Ovadia, R., Dept. of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
De Jong, W., Department of Plant Breeding, Cornell University, 252 Emerson Hall, Ithaca, NY 14853, United States
Paran, I., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
23
To page:
29
(
Total pages:
7
)
Abstract:
Pepper plants containing the dominant A gene accumulate anthocyanin pigments in the foliage, flower and immature fruit. We previously mapped A to pepper chromosome 10 in the F2 progeny of a cross between 5226 (purple-fruited) and PI 159234 (green-fruited) to a region that corresponds, in tomato, to the location of Petunia anthocyanin 2 (An2), a regulator of anthocyanin biosynthesis. This suggested that A encodes a homologue of Petunia An2. Using the sequences of An2 and a corresponding tomato expressed sequence tag, we isolated a pepper cDNA orthologous to An2 that cosegregated with A. We subsequently determined the expression of A by Northern analysis, using RNA extracted from fruits, flowers and leaves of 5226 and PI 159234. In 5226, expression was detected in all stages of fruit development and in both flower and leaf. In contrast, A was not expressed in the sampled tissues in PI 159234. Genomic sequence comparison of A between green- and purple-fruited genotypes revealed no differences in the coding region, indicating that the lack of expression of A in the green genotypes can be attributed to variation in the promoter region. By analyzing the expression of the structural genes in the anthocyanin biosynthetic pathway in 5226 and PI 159234, it was determined that, similar to Petunia, the early genes in the pathway are regulated independently of A, while expression of the late genes is A-dependent. © Springer-Verlag 2004.
Note:
Related Files :
chromosome mapping
DNA
gene expression
genetic engineering
Genetics
Petunia
pigmentation
RNA
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-004-1625-9
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24141
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
Scientific Publication
The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia
109
Borovsky, Y., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Oren-Shamir, M., Dept. of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Ovadia, R., Dept. of Ornamental Horticulture, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
De Jong, W., Department of Plant Breeding, Cornell University, 252 Emerson Hall, Ithaca, NY 14853, United States
Paran, I., Dept. of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
The A locus that controls anthocyanin accumulation in pepper encodes a MYB transcription factor homologous to Anthocyanin2 of Petunia
Pepper plants containing the dominant A gene accumulate anthocyanin pigments in the foliage, flower and immature fruit. We previously mapped A to pepper chromosome 10 in the F2 progeny of a cross between 5226 (purple-fruited) and PI 159234 (green-fruited) to a region that corresponds, in tomato, to the location of Petunia anthocyanin 2 (An2), a regulator of anthocyanin biosynthesis. This suggested that A encodes a homologue of Petunia An2. Using the sequences of An2 and a corresponding tomato expressed sequence tag, we isolated a pepper cDNA orthologous to An2 that cosegregated with A. We subsequently determined the expression of A by Northern analysis, using RNA extracted from fruits, flowers and leaves of 5226 and PI 159234. In 5226, expression was detected in all stages of fruit development and in both flower and leaf. In contrast, A was not expressed in the sampled tissues in PI 159234. Genomic sequence comparison of A between green- and purple-fruited genotypes revealed no differences in the coding region, indicating that the lack of expression of A in the green genotypes can be attributed to variation in the promoter region. By analyzing the expression of the structural genes in the anthocyanin biosynthetic pathway in 5226 and PI 159234, it was determined that, similar to Petunia, the early genes in the pathway are regulated independently of A, while expression of the late genes is A-dependent. © Springer-Verlag 2004.
Scientific Publication
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