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פותח על ידי קלירמאש פתרונות בע"מ -
The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning
Year:
2013
Source of publication :
New Phytologist
Authors :
איזיקסון, טל
;
.
אלקן, נעם
;
.
פרוסקי, דב
;
.
Volume :
197
Co-Authors:
Shi, J.X., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel, National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Alkan, N., Department of Postharvest Science of Fresh Produce, ARO the Volcani Center, Po Box 6 Bet-Dagan 50250, Bet-Dagan, Israel
He, Y., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Lashbrooke, J., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Matas, A.J., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Meir, S., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Malitsky, S., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Isaacson, T., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO the Volcani Center, Po Box 6 Bet-Dagan 50250, Bet-Dagan, Israel
Leshkowitz, D., Department of Biological Services, Weizmann Institute of Science, Po Box 26 Rehovot 76100, Rehovot, Israel
Schreiber, L., Department of Ecophysiology, University of Bonn, Kirschallee 1, Bonn 53115, Germany
Granell, A.R., Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Valencia, 46022, Spain
Widemann, E., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Grausem, B., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Pinot, F., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Rose, J.K.C., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Rothan, C., UMR 619 Fruit Biology, INRA Bordeaux, 71 Av. Edouard Bourleaux, Villenave d'Ornon, 33 883, France
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Facilitators :
From page:
468
To page:
480
(
Total pages:
13
)
Abstract:
Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato SlSHINE3 (SlSHN3) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in Arabidopsis phenocopied overexpression of the Arabidopsis SHNs. Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNAi lines, mutation in the SlSHN3 target gene, SlCYP86A69, resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH-dependent ω-hydroxylation activity, particularly of C18:1 fatty acid to the 18-hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning. © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.
Note:
Related Files :
arabidopsis
gene expression
gene silencing
Genetics
molecular genetics
mutation
phenotype
Solanum lycopersicum
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/nph.12032
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24219
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
You may also be interested in
Scientific Publication
The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning
197
Shi, J.X., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel, National Center for Molecular Characterization of Genetically Modified Organisms, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China
Adato, A., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Alkan, N., Department of Postharvest Science of Fresh Produce, ARO the Volcani Center, Po Box 6 Bet-Dagan 50250, Bet-Dagan, Israel
He, Y., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Lashbrooke, J., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Matas, A.J., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Meir, S., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Malitsky, S., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Isaacson, T., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO the Volcani Center, Po Box 6 Bet-Dagan 50250, Bet-Dagan, Israel
Leshkowitz, D., Department of Biological Services, Weizmann Institute of Science, Po Box 26 Rehovot 76100, Rehovot, Israel
Schreiber, L., Department of Ecophysiology, University of Bonn, Kirschallee 1, Bonn 53115, Germany
Granell, A.R., Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia, Valencia, 46022, Spain
Widemann, E., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Grausem, B., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Pinot, F., Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, Strasbourg Cedex, 67083, France
Rose, J.K.C., Department of Plant Biology, Cornell University, Ithaca, NY 14853, United States
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
Rothan, C., UMR 619 Fruit Biology, INRA Bordeaux, 71 Av. Edouard Bourleaux, Villenave d'Ornon, 33 883, France
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Po Box 26, Rehovot 76100, Israel
The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning
Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato SlSHINE3 (SlSHN3) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in Arabidopsis phenocopied overexpression of the Arabidopsis SHNs. Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNAi lines, mutation in the SlSHN3 target gene, SlCYP86A69, resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH-dependent ω-hydroxylation activity, particularly of C18:1 fatty acid to the 18-hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning. © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.
Scientific Publication
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