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פותח על ידי קלירמאש פתרונות בע"מ -
Testicular amplificaion and impaired transmission of human butyrylcholinesterase cDNA in transgenic mice
Year:
1994
Source of publication :
Human Reproduction
Authors :
שני, משה
;
.
Volume :
9
Co-Authors:
Beeri, R., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Gnatt, A., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Lapidot-lifson, Y., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel, Department of Obstetrics and Gynaecology, The Sackler Faculty of Medicine, Tel-Aviv University, Edith Wolfson Medical Centre, Holon (58100), Israel
Ginzberg, D., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Shani, M., Department of Genetic Engineering, Institute of Animal Science, Agricultural Research, 906, Beit Dagan 50250, Israel
Soreq, H., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Zakut, H., Department of Obstetrics and Gynaecology, The Sackler Faculty of Medicine, Tel-Aviv University, Edith Wolfson Medical Centre, Holon (58100), Israel
Facilitators :
From page:
284
To page:
292
(
Total pages:
9
)
Abstract:
Gene amplification occurs frequently in tumour tissues yet is, in general, non-inheritable. To study the molecular mechanisms conferring this restraint, we created transgenic mice carrying a human butyrylcholinesterase (BCHE) coding sequence, previously found to be amplified in a father and son. Blot hybridization of tail DNA samples revealed somatic transgene amplifications with variable restriction patterns and intensities, suggesting the occurrence of independent amplification events, in 31% (11/35) of mice from the FII generation but in only 3.5% (2/58) of the FII and FIV generations. In contrast, >10-fold amplifications of the BCHE transgene and the endogenous acetylcholinesterase and c-raf genes appeared in both testis and epididymis DNA from >80% of FIII mice. Drastic, selective reductions in testis BCHEmRNA but not in actin mRNA were detected by the PCR amplification of testis cDNA from the transgenic mice, and apparently resulted in the limited transmission of amplified genes. The testicular amplification of the BCHE transgene may potentially represent a general phenomenon with clinical implications in human infertility. © 1994 Oxford University Press.
Note:
Related Files :
Acetylcholinesterase
Animal
gene amplification
gene transfer
Male
mice
testis
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24230
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
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Scientific Publication
Testicular amplificaion and impaired transmission of human butyrylcholinesterase cDNA in transgenic mice
9
Beeri, R., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Gnatt, A., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Lapidot-lifson, Y., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel, Department of Obstetrics and Gynaecology, The Sackler Faculty of Medicine, Tel-Aviv University, Edith Wolfson Medical Centre, Holon (58100), Israel
Ginzberg, D., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Shani, M., Department of Genetic Engineering, Institute of Animal Science, Agricultural Research, 906, Beit Dagan 50250, Israel
Soreq, H., Department of Biological Chemistry, Life Sciences Institute, Hebrew University, Jerusalem 91904, Israel
Zakut, H., Department of Obstetrics and Gynaecology, The Sackler Faculty of Medicine, Tel-Aviv University, Edith Wolfson Medical Centre, Holon (58100), Israel
Testicular amplificaion and impaired transmission of human butyrylcholinesterase cDNA in transgenic mice
Gene amplification occurs frequently in tumour tissues yet is, in general, non-inheritable. To study the molecular mechanisms conferring this restraint, we created transgenic mice carrying a human butyrylcholinesterase (BCHE) coding sequence, previously found to be amplified in a father and son. Blot hybridization of tail DNA samples revealed somatic transgene amplifications with variable restriction patterns and intensities, suggesting the occurrence of independent amplification events, in 31% (11/35) of mice from the FII generation but in only 3.5% (2/58) of the FII and FIV generations. In contrast, >10-fold amplifications of the BCHE transgene and the endogenous acetylcholinesterase and c-raf genes appeared in both testis and epididymis DNA from >80% of FIII mice. Drastic, selective reductions in testis BCHEmRNA but not in actin mRNA were detected by the PCR amplification of testis cDNA from the transgenic mice, and apparently resulted in the limited transmission of amplified genes. The testicular amplification of the BCHE transgene may potentially represent a general phenomenon with clinical implications in human infertility. © 1994 Oxford University Press.
Scientific Publication
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