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פותח על ידי קלירמאש פתרונות בע"מ -
Expression of small heat-shock proteins at low temperatures: A possible role in protecting against chilling injuries
Year:
1998
Authors :
לוריא, סוזן
;
.
Volume :
117
Co-Authors:
Sabehat, A., Kennedy-Leigh Ctr. for Horticulture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lurie, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Weiss, D., Kennedy-Leigh Ctr. for Horticulture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Facilitators :
From page:
651
To page:
658
(
Total pages:
8
)
Abstract:
We previously reported that short exposure of tomato (Lycopersicon esculentum L.) fruits to high temperature protects them from chilling injury. To study the involvement of heat-shock proteins (HSPs) in the acquisition of low-temperature tolerance, we cloned two heat-shock-induced genes that are also expressed at low temperatures. The cloned cDNAs belong to the small HSP group. Sequence analyses of the clones showed perfect homology to the tomato-ripening gene tom66 and to the tomato chloroplaslic HSP21 gene tom111. The expression of both genes was induced by high temperature in fruits, flowers, leaves, and stems, but not by low or ambient temperatures or by other stresses such as drought and anaerobic conditions. When the heated fruits were transferred to low temperature, tom66 and tom111 mRNA levels first decreased but were then reinduced. Induction was not observed in nonheated fruits at low temperature. Immunodetection of tom111-encoded protein indicated that this protein is present at low temperatures in the heated fruits. The results of this study show that the expression of tom66 and tom111 is correlated with protection against some, but not all, symptoms of chilling injury.
Note:
Related Files :
biosynthesis
Chloroplasts
Cloning, Molecular
Cold
gene expression regulation
Heat-Shock Proteins
metabolism
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24247
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
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Scientific Publication
Expression of small heat-shock proteins at low temperatures: A possible role in protecting against chilling injuries
117
Sabehat, A., Kennedy-Leigh Ctr. for Horticulture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Lurie, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Weiss, D., Kennedy-Leigh Ctr. for Horticulture, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel
Expression of small heat-shock proteins at low temperatures: A possible role in protecting against chilling injuries
We previously reported that short exposure of tomato (Lycopersicon esculentum L.) fruits to high temperature protects them from chilling injury. To study the involvement of heat-shock proteins (HSPs) in the acquisition of low-temperature tolerance, we cloned two heat-shock-induced genes that are also expressed at low temperatures. The cloned cDNAs belong to the small HSP group. Sequence analyses of the clones showed perfect homology to the tomato-ripening gene tom66 and to the tomato chloroplaslic HSP21 gene tom111. The expression of both genes was induced by high temperature in fruits, flowers, leaves, and stems, but not by low or ambient temperatures or by other stresses such as drought and anaerobic conditions. When the heated fruits were transferred to low temperature, tom66 and tom111 mRNA levels first decreased but were then reinduced. Induction was not observed in nonheated fruits at low temperature. Immunodetection of tom111-encoded protein indicated that this protein is present at low temperatures in the heated fruits. The results of this study show that the expression of tom66 and tom111 is correlated with protection against some, but not all, symptoms of chilling injury.
Scientific Publication
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