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פותח על ידי קלירמאש פתרונות בע"מ -
Isolation of a thioredoxin h cDNA from grapefruit peel tissue that is induced upon infection by Penicillium digitatum and elicitation of pathogen resistance
Year:
2004
Authors :
דרובי, סמיר
;
.
חסדאי, מ'
;
.
פורת, רון
;
.
Volume :
65
Co-Authors:
Hasdai, M., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Elmaci, C., Department of Animal Science, Uludag University, Gorukle Campus, 16059 Bursa, Turkey
Goldschmidt, E.E., Faculty of Agricultural, Food and Environmental Quality Sciences, Institute for Plant Sciences and Genetics, Hebrew University of Jerusalem, Rehovot 76100, Israel
Droby, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
277
To page:
283
(
Total pages:
7
)
Abstract:
Thioredoxins are ubiquitous small proteins with a redox-active disulfide bridge; they participate in redox regulation of selected target proteins, such as transcription factors, receptors and various metabolic and regulatory enzymes. In the present study, we isolated by PCR cDNA subtraction analysis a grapefruit 135-bp polymerase chain reaction (PCR) fragment whose expression was enhanced in the fruit peel tissue following the application of Candida oleophila yeast cells, an elicitation treatment that induces resistance towards the green mold pathogen Penicillium digitatum (Pers.:Fr.) Sacc., and that showed high sequence homology with various thioredoxin h (TRXh) genes. The full-length cDNA sequence of cTRXh (for citrus TRXh) was further isolated by screening a grapefruit flavedo cDNA library; it had a total length of 785 bp with an open reading frame of 369 bp, and encoded a predicted polypeptide of 123 amino acids with a molecular mass of 13.4 kDa. Expression analysis studies revealed that cTRXh mRNA levels increased in grapefruit peel tissue upon infection by P. digitatum, and following the elicitation of fruit disease resistance by application of C. oleophila yeast cells, wounding, exposure to ethylene, and a hot water rinsing and brushing treatment. These results indicate that cTRXh may be part of the molecular mechanisms involved in the induction of pathogen defense responses in grapefruit, and may be involved in the signaling cascades leading to enhanced resistance. © 2005 Elsevier Ltd. All rights reserved.
Note:
Related Files :
candida
Candida oleophila
Citrus
Citrus x paradisi
grapefruits
Pathogen resistance
Penicillium digitatum
Thioredoxin
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.pmpp.2005.02.009
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24248
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
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Scientific Publication
Isolation of a thioredoxin h cDNA from grapefruit peel tissue that is induced upon infection by Penicillium digitatum and elicitation of pathogen resistance
65
Hasdai, M., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Elmaci, C., Department of Animal Science, Uludag University, Gorukle Campus, 16059 Bursa, Turkey
Goldschmidt, E.E., Faculty of Agricultural, Food and Environmental Quality Sciences, Institute for Plant Sciences and Genetics, Hebrew University of Jerusalem, Rehovot 76100, Israel
Droby, S., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Porat, R., Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Isolation of a thioredoxin h cDNA from grapefruit peel tissue that is induced upon infection by Penicillium digitatum and elicitation of pathogen resistance
Thioredoxins are ubiquitous small proteins with a redox-active disulfide bridge; they participate in redox regulation of selected target proteins, such as transcription factors, receptors and various metabolic and regulatory enzymes. In the present study, we isolated by PCR cDNA subtraction analysis a grapefruit 135-bp polymerase chain reaction (PCR) fragment whose expression was enhanced in the fruit peel tissue following the application of Candida oleophila yeast cells, an elicitation treatment that induces resistance towards the green mold pathogen Penicillium digitatum (Pers.:Fr.) Sacc., and that showed high sequence homology with various thioredoxin h (TRXh) genes. The full-length cDNA sequence of cTRXh (for citrus TRXh) was further isolated by screening a grapefruit flavedo cDNA library; it had a total length of 785 bp with an open reading frame of 369 bp, and encoded a predicted polypeptide of 123 amino acids with a molecular mass of 13.4 kDa. Expression analysis studies revealed that cTRXh mRNA levels increased in grapefruit peel tissue upon infection by P. digitatum, and following the elicitation of fruit disease resistance by application of C. oleophila yeast cells, wounding, exposure to ethylene, and a hot water rinsing and brushing treatment. These results indicate that cTRXh may be part of the molecular mechanisms involved in the induction of pathogen defense responses in grapefruit, and may be involved in the signaling cascades leading to enhanced resistance. © 2005 Elsevier Ltd. All rights reserved.
Scientific Publication
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