חיפוש מתקדם
Planta
Fridlender, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Lev-Yadun, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel, Zinman Institute of Archaeology, University of Haifa, Haifa 31905, Israel
Baburek, I., Institute of Experimental Botany, Acad. of Sci. of the Czech Republic, Na Karlovce 1, 160 00 Praha 6, Czech Republic
Angelis, K., Institute of Experimental Botany, Acad. of Sci. of the Czech Republic, Na Karlovce 1, 160 00 Praha 6, Czech Republic
Levy, A.A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Post-germinative proliferation of cells was studied in cotyledons of Nicotiana tabucum L., Petunia hybrida Vilm. and Arabidopsis thaliana (L.) Heynh. Patterns of cell divisions after germination were characterized by clonal analysis in cotyledons of N. tabacum. The fate of initial cells, which are formed by the end of embryogenesis, was quite variable: cells could undergo between one to seven, and most often, between three to five anticlinal divisions after germination. Sector shape suggested that there were more divisions in length than in width, particularly at the periphery of the cotyledon. The boundaries of clones generated by irradiation of mature seeds did not intersect the midvein, and in most cases, did not intersect lateral veins. The time course of cell divisions during post- germinative development was analyzed cytologically in cotyledons of N. tabacum and P. hybrida. No divisions were detected up to the second day after sowing (DAS), when the radicle emerged. Cotyledon cells started to divide at a rapid rate between 2 and 3 DAS, reaching a mitotic index of about 2% at 3 4 DAS. A rapid decline followed the peak, and no divisions were detected 6 7 DAS. Similarities between leaf and cotyledon development are discussed. In addition, we show that divisions in cotyledons of N. tabacum and A. thaliana chlorophyll mutants can be exploited for a quick and sensitive bioassay from which the effects of various mutagens and DNA repair genes can be assessed.
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Cell divisions in cotyledons after germination: Localization, time course and utilization for a mutagenesis assay
199
Fridlender, M., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Lev-Yadun, S., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel, Zinman Institute of Archaeology, University of Haifa, Haifa 31905, Israel
Baburek, I., Institute of Experimental Botany, Acad. of Sci. of the Czech Republic, Na Karlovce 1, 160 00 Praha 6, Czech Republic
Angelis, K., Institute of Experimental Botany, Acad. of Sci. of the Czech Republic, Na Karlovce 1, 160 00 Praha 6, Czech Republic
Levy, A.A., Department of Plant Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Cell divisions in cotyledons after germination: Localization, time course and utilization for a mutagenesis assay
Post-germinative proliferation of cells was studied in cotyledons of Nicotiana tabucum L., Petunia hybrida Vilm. and Arabidopsis thaliana (L.) Heynh. Patterns of cell divisions after germination were characterized by clonal analysis in cotyledons of N. tabacum. The fate of initial cells, which are formed by the end of embryogenesis, was quite variable: cells could undergo between one to seven, and most often, between three to five anticlinal divisions after germination. Sector shape suggested that there were more divisions in length than in width, particularly at the periphery of the cotyledon. The boundaries of clones generated by irradiation of mature seeds did not intersect the midvein, and in most cases, did not intersect lateral veins. The time course of cell divisions during post- germinative development was analyzed cytologically in cotyledons of N. tabacum and P. hybrida. No divisions were detected up to the second day after sowing (DAS), when the radicle emerged. Cotyledon cells started to divide at a rapid rate between 2 and 3 DAS, reaching a mitotic index of about 2% at 3 4 DAS. A rapid decline followed the peak, and no divisions were detected 6 7 DAS. Similarities between leaf and cotyledon development are discussed. In addition, we show that divisions in cotyledons of N. tabacum and A. thaliana chlorophyll mutants can be exploited for a quick and sensitive bioassay from which the effects of various mutagens and DNA repair genes can be assessed.
Scientific Publication
You may also be interested in