חיפוש מתקדם
Microbiology
Manulis, S., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Shafrir, H., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Epstein, E., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Lichter, A., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Barash, I., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Various Streptomyces spp. including S. violaceus, S. scabies, S. griseus, S. exfoliatus, S. coelicolor and S. lividans secrete indole-3-acetic acid (IAA) when fed with L-tryptophan (Trp). Production of IAA was detected in Streptomyces strains causing potato scab as well as in non-pathogenic strains. The pathways for IAA synthesis from Trp were investigated in S. violaceus and S. exfoliatus. Indole-3-acetamide (IAM), indole-3-lactic acid (ILA), indole-3-ethanol (IEt) and IAA were identified by HPLC and GC-MS. Streptomyces cells were capable of catabolizing IAM, ILA, IEt and indole-3-acetaldehyde (IAAld) into IAA. Incorporation of radioactivity into IAM, IAA and IAL but not IEt was detected when cells were fed with L-[3-14C]tryptophan. Results indicate the presence of the IAM pathway (Trp → IAM → IAA) and the possible presence of additional pathways for IAA biosynthesis in Streptomyces.
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תנאי שימוש
Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.
140
Manulis, S., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Shafrir, H., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Epstein, E., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Lichter, A., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Barash, I., Department of Botany, Tel Aviv University, Tel Aviv 69978, Israel
Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.
Various Streptomyces spp. including S. violaceus, S. scabies, S. griseus, S. exfoliatus, S. coelicolor and S. lividans secrete indole-3-acetic acid (IAA) when fed with L-tryptophan (Trp). Production of IAA was detected in Streptomyces strains causing potato scab as well as in non-pathogenic strains. The pathways for IAA synthesis from Trp were investigated in S. violaceus and S. exfoliatus. Indole-3-acetamide (IAM), indole-3-lactic acid (ILA), indole-3-ethanol (IEt) and IAA were identified by HPLC and GC-MS. Streptomyces cells were capable of catabolizing IAM, ILA, IEt and indole-3-acetaldehyde (IAAld) into IAA. Incorporation of radioactivity into IAM, IAA and IAL but not IEt was detected when cells were fed with L-[3-14C]tryptophan. Results indicate the presence of the IAM pathway (Trp → IAM → IAA) and the possible presence of additional pathways for IAA biosynthesis in Streptomyces.
Scientific Publication
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