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Metabolism of 26‐[14C]hydroxyecdysone 26‐phosphate in the tobacco hornworm, Manduca sexta L., to a new ecdysteroid conjugate: 26‐[14C]hydroxyecdysone 22‐glucoside
Year:
1987
Authors :
סבובודה, ג'יימס
;
.
Volume :
4
Co-Authors:
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Feldlaufer, M.F., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Rees, H.H., Department of Biochemistry, University of Liverpool, Liverpool, United Kingdom
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Wilzer, K.R., Jr., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Facilitators :
From page:
1
To page:
15
(
Total pages:
15
)
Abstract:
Following injection into female Manduca sexta pupae, [14C]cholesterol is converted to a radiolabeled C21 nonecdysteroid conjugate as well as ecdysteroid conjugates, which in ovaries and newly‐laid eggs consist mainly of labeled 26‐hydroxyecdysone 26‐phosphate. During embryogenesis, as the level of 26‐hydroxyecdysone 26‐phosphate decreases there is a concurrent increase in the amount of a new, labeled ecdysteroid conjugate. This conjugate, which is the major ecdysteroid conjugate (9.4 μg/g) in 0‐ to 1‐hour‐old larvae was identified as 26‐hydroxyecdysone 22‐glucoside by nuclear magnetic resonance and chemical ionization mass spectrometry. This is the first ecdysteroid glucoside to be identified from an insect. The disappearance of 26‐hydroxyecdysone 26‐phosphate in 0‐ to 1‐hour‐old larvae indicates that the 26‐hydroxyecdysone 22‐glucoside is derived from 26‐hydroxyecdysone 26‐phosphate. 3‐Epi‐26‐hydroxyecdysone was the major free ecdysteroid isolated from these larvae and 3‐epi‐20,26‐dihydroxyecdysone was the next most abundant ecdysteroid isolated. Interestingly, the 0‐ to 1‐hour‐old larvae contained the highest levels of 3α‐ecdysteroids per gram of insect tissue (8.7 μg/g) to be isolated from an insect, yet there was a complete absence of the corresponding free 3β‐epimers. The ecdysteroid conjugate profiles of ovaries and 0‐ to 1‐hour‐old larvae are discussed. Methodology is presented that permits the efficient separation of free and conjugated ecdysteroids and nonecdysteroid conjugates (C21‐steroid conjugates). Copyright © 1987 Wiley‐Liss, Inc.
Note:
Related Files :
3‐epi‐20,26‐dihydroxyecdysone
26‐hydroxyecdysone 26‐phosphate
radiolabeled ecdysteroid conjugates
עוד תגיות
תוכן קשור
More details
DOI :
10.1002/arch.940040102
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24270
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
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Scientific Publication
Metabolism of 26‐[14C]hydroxyecdysone 26‐phosphate in the tobacco hornworm, Manduca sexta L., to a new ecdysteroid conjugate: 26‐[14C]hydroxyecdysone 22‐glucoside
4
Thompson, M.J., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Feldlaufer, M.F., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Lozano, R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Rees, H.H., Department of Biochemistry, University of Liverpool, Liverpool, United Kingdom
Lusby, W.R., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Svoboda, J.A., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Wilzer, K.R., Jr., Insect and Nematode Hormone Laboratory, Agricultural Research Service, Usda, Beltsville, Maryland, United States
Metabolism of 26‐[14C]hydroxyecdysone 26‐phosphate in the tobacco hornworm, Manduca sexta L., to a new ecdysteroid conjugate: 26‐[14C]hydroxyecdysone 22‐glucoside
Following injection into female Manduca sexta pupae, [14C]cholesterol is converted to a radiolabeled C21 nonecdysteroid conjugate as well as ecdysteroid conjugates, which in ovaries and newly‐laid eggs consist mainly of labeled 26‐hydroxyecdysone 26‐phosphate. During embryogenesis, as the level of 26‐hydroxyecdysone 26‐phosphate decreases there is a concurrent increase in the amount of a new, labeled ecdysteroid conjugate. This conjugate, which is the major ecdysteroid conjugate (9.4 μg/g) in 0‐ to 1‐hour‐old larvae was identified as 26‐hydroxyecdysone 22‐glucoside by nuclear magnetic resonance and chemical ionization mass spectrometry. This is the first ecdysteroid glucoside to be identified from an insect. The disappearance of 26‐hydroxyecdysone 26‐phosphate in 0‐ to 1‐hour‐old larvae indicates that the 26‐hydroxyecdysone 22‐glucoside is derived from 26‐hydroxyecdysone 26‐phosphate. 3‐Epi‐26‐hydroxyecdysone was the major free ecdysteroid isolated from these larvae and 3‐epi‐20,26‐dihydroxyecdysone was the next most abundant ecdysteroid isolated. Interestingly, the 0‐ to 1‐hour‐old larvae contained the highest levels of 3α‐ecdysteroids per gram of insect tissue (8.7 μg/g) to be isolated from an insect, yet there was a complete absence of the corresponding free 3β‐epimers. The ecdysteroid conjugate profiles of ovaries and 0‐ to 1‐hour‐old larvae are discussed. Methodology is presented that permits the efficient separation of free and conjugated ecdysteroids and nonecdysteroid conjugates (C21‐steroid conjugates). Copyright © 1987 Wiley‐Liss, Inc.
Scientific Publication
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