Johnson, A.J., USDA-ARS Crop Production and Pest Control Research Unit, Department of Entomology, Purdue University, West Lafayette, IN, United States
Shukle, R.H., USDA-ARS Crop Production and Pest Control Research Unit, Department of Entomology, Purdue University, West Lafayette, IN, United States
Chen, M.-S., USDA-ARS Hard Winter Wheat Genetics Research Unit, Manhattan, KS, United States, Department of Entomology, Kansas State University, Manhattan, KS, United States
Srivastava, S., Department of Statistics, Duke University, Durham, NC, United States
Subramanyam, S., Department of Agronomy, Purdue University, West Lafayette, IN, United States
Schemerhorn, B.J., USDA-ARS Crop Production and Pest Control Research Unit, Department of Entomology, Purdue University, West Lafayette, IN, United States
Weintraub, P.G., Agricultural Research Organization Gilat Research Center, D.N. Negev, Israel, Department of Zoology, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abdel Moniem, H.E.M., Department of Entomology, Purdue University, West Lafayette, IN, United States
Flanders, K.L., Department of Entomology and Plant Pathology, Auburn University, Auburn, AL, United States
Buntin, G.D., Department of Entomology, University of Georgia College of Agriculture Experiment Stations, Georgia Station, Griffin, GA, United States
Williams, C.E., USDA-ARS Crop Production and Pest Control Research Unit, Department of Entomology, Purdue University, West Lafayette, IN, United States

Evidence is emerging that some proteins secreted by gall-forming parasites of plants act as effectors responsible for systemic changes in the host plant, such as galling and nutrient tissue formation. A large number of secreted salivary gland proteins (SSGPs) that are the putative effectors responsible for the physiological changes elicited in susceptible seedling wheat by Hessian fly, Mayetiola destructor (Say), larvae have been documented. However, how the genes encoding these candidate effectors might respond under field conditions is unknown. The goal of this study was to use microarray analysis to investigate variation in SSGP transcript abundance amongst field collections from different geographical regions (southeastern USA, central USA, and the Middle East). Results revealed significant variation in SSGP transcript abundance amongst the field collections studied. The field collections separated into three distinct groups that corresponded to the wheat classes grown in the different geographical regions as well as to recently described Hessian fly populations. These data support previous reports correlating Hessian fly population structure with micropopulation differences owing to agro-ecosystem parameters such as cultivation of regionally adapted wheat varieties, deployment of resistance genes and variation in climatic conditions. © Published 2014. This article is a U.S. Government work and is in the public domain in the USA.