אסיף מאגר המחקר החקלאי

Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco

Year:

2014

Source of publication :

Authors :

Volume :

14

Co-Authors:

Shukla, P., Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
Singh, N.K., Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
Kumar, D., Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
Vijayan, S., Pondicherry Biotech Private Limited, I.T. Park, Pondicherry Engineering College Campus, Puducherry, Pillaichavady 605 014, India
Ahmed, I., Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India
Kirti, P.B., Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India

Facilitators :

Link :

Abstract:

Usable male sterility systems have immense potential in developing hybrid varieties in crop plants, which can also be used as a biological safety containment to prevent horizontal transgene flow. Barnase-Barstar system developed earlier was the first approach to engineer male sterility in plants. In an analogous situation, we have evolved a system of inducing pollen abortion and male sterility in transgenic tobacco by expressing a plant gene coding for a protein with known developmental function in contrast to the Barnase-Barstar system, which deploys genes of prokaryotic origin, i.e., from Bacillus amyloliquefaciens. We have used a plant pathogen-induced gene, cysteine protease for inducing male sterility. This gene was identified in the wild peanut, Arachis diogoi differentially expressed when it was challenged with the late leaf spot pathogen, Phaeoisariopsis personata. Arachis diogoi cysteine protease (AdCP) was expressed under the strong tapetum-specific promoter (TA29) and tobacco transformants were generated. Morphological and histological analysis of AdCP transgenic plants showed ablated tapetum and complete pollen abortion in three transgenic lines. Furthermore, transcript analysis displayed the expression of cysteine protease in these male sterile lines and the expression of the protein was identified in western blot analysis using its polyclonal antibody raised in the rabbit system. © 2014 Springer-Verlag.

Note:

Related Files :

תוכן קשור

More details

You may also be interested in