Co-Authors:
Boer, P., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Lipstein, B., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
De Vries, A., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Sperling, O., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Abstract:
The effect of increasing cellular ribose 5-phosphate (ribose-5-P) availability by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on the rate of 5-phosphoribosyl-1-pyrophosphate (P-ribose-PP) generation, was studied in slices of rat liver at varying Pi concentration. It was found that at Pi concentration prevailing in the tissue at extracellular physiological Pi concentration, ribose-5-P availability is saturating for P-ribose-PP generation, as gauged by the rate of adenine incorporation into tissue nucleotides. The effect of altering P-ribose-PP availability on the rate of de novo purine production gauged by the rate of formate incorporation into purines, was also studied. It was found that the physiological P-ribose-PP concentration in rat liver tissue is limiting for purine synthesis de novo. Depletion of cellular P-ribose-PP, achieved by increase of P-ribose-PP consumption, decelerated purine synthesis, while increase of P-ribose-PP availability, achieved by activation of P-ribose-PP synthetase occurring at elevated Pi concentration, resulted in acceleration of purine synthesis. © 1976.
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