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Journal of Biotechnology
Kauffmann, C.G., Institute of Soils and Water, Volcani Research Institute, 50250 Bet Dagan, Israel
Mandelbaum, R.T., Institute of Soils and Water, Volcani Research Institute, 50250 Bet Dagan, Israel
Cell-free crude extracts of Pseudomonas sp. strain ADP (ADP) that could rapidly degrade atrazine were successfully entrapped in a tetramethyl orthosilicate (TMOS) sol-gel. The immobilized crude extract maintained atrazine-degrading activity for at least a few days. However, compared with the non-entrapped crude extract, the sol-gel immobilization resulted in significant loss of activity. Part of the loss in activity is due to methanol produced during gel formation.Cell-free crude extracts of Pseudomonas sp. strain ADP (ADP) that could rapidly degrade atrazine were successfully entrapped in a tetramethyl orthosilicate (TMOS) sol-gel. The immobilized crude extract maintained atrazine-degrading activity for at least a few days. However, compared with the non-entrapped crude extract, the sol-gel immobilization resulted in significant loss of activity. Part of the loss in activity is due to methanol produced during gel formation.
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תנאי שימוש
Entrapment of atrazine-degrading enzymes in sol-gel glass
51
Kauffmann, C.G., Institute of Soils and Water, Volcani Research Institute, 50250 Bet Dagan, Israel
Mandelbaum, R.T., Institute of Soils and Water, Volcani Research Institute, 50250 Bet Dagan, Israel
Entrapment of atrazine-degrading enzymes in sol-gel glass
Cell-free crude extracts of Pseudomonas sp. strain ADP (ADP) that could rapidly degrade atrazine were successfully entrapped in a tetramethyl orthosilicate (TMOS) sol-gel. The immobilized crude extract maintained atrazine-degrading activity for at least a few days. However, compared with the non-entrapped crude extract, the sol-gel immobilization resulted in significant loss of activity. Part of the loss in activity is due to methanol produced during gel formation.Cell-free crude extracts of Pseudomonas sp. strain ADP (ADP) that could rapidly degrade atrazine were successfully entrapped in a tetramethyl orthosilicate (TMOS) sol-gel. The immobilized crude extract maintained atrazine-degrading activity for at least a few days. However, compared with the non-entrapped crude extract, the sol-gel immobilization resulted in significant loss of activity. Part of the loss in activity is due to methanol produced during gel formation.
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