חיפוש מתקדם
Animal Genetics
Levin, I., Laboratory of Animal Breeding and Genetics, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Cheng, H.H., USDA, Avian Disease and Oncology Laboratory, Agricultural Research Service, 3606 East Mount Hope Road, East Lansing, Michigan, 48823, United States
Baxter‐Jones, C., PO Box 727, Lewisburg, West Virginia, 24901, United States
Hillel, J., Laboratory of Animal Breeding and Genetics, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Forty‐eight primer‐pairs complementary to unique DNA sequences flanking chicken (genus Gallus) genomic (TG)n microsatellite repeats were previously designed. These primer‐pairs were used in the polymerase chain reaction to amplify turkey (genus Meleagris) genomic DNA loci. Results indicated that the majority (92%) of these primer‐pairs generated amplification products in turkey genomic DNA. Hybridization using end‐labelled (TG)8 as a probe showed that, out of 41 primer‐pairs tested, only 14 generated an amplification product that also contained a detectable (TG)n microsatellite repeat when turkey DNA was the template. Among 18 primerpairs tested for polymorphism, using three commercial turkey lines, five were found to exhibit length polymorphism, three of which did not contain a detectable TG repeat. Therefore, a significant portion of chicken microsatellite markers can be useful for genomic mapping and linkage analysis in the turkey, reducing the costs involved in producing turkey‐specific microsatellite markers. © 1995 International Society for Animal Genetics
פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Turkey microsatellite DNA loci amplified by chicken‐specific primers
26
Levin, I., Laboratory of Animal Breeding and Genetics, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Cheng, H.H., USDA, Avian Disease and Oncology Laboratory, Agricultural Research Service, 3606 East Mount Hope Road, East Lansing, Michigan, 48823, United States
Baxter‐Jones, C., PO Box 727, Lewisburg, West Virginia, 24901, United States
Hillel, J., Laboratory of Animal Breeding and Genetics, The Hebrew University of Jerusalem, PO Box 12, Rehovot, 76100, Israel
Turkey microsatellite DNA loci amplified by chicken‐specific primers
Forty‐eight primer‐pairs complementary to unique DNA sequences flanking chicken (genus Gallus) genomic (TG)n microsatellite repeats were previously designed. These primer‐pairs were used in the polymerase chain reaction to amplify turkey (genus Meleagris) genomic DNA loci. Results indicated that the majority (92%) of these primer‐pairs generated amplification products in turkey genomic DNA. Hybridization using end‐labelled (TG)8 as a probe showed that, out of 41 primer‐pairs tested, only 14 generated an amplification product that also contained a detectable (TG)n microsatellite repeat when turkey DNA was the template. Among 18 primerpairs tested for polymorphism, using three commercial turkey lines, five were found to exhibit length polymorphism, three of which did not contain a detectable TG repeat. Therefore, a significant portion of chicken microsatellite markers can be useful for genomic mapping and linkage analysis in the turkey, reducing the costs involved in producing turkey‐specific microsatellite markers. © 1995 International Society for Animal Genetics
Scientific Publication
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