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פותח על ידי קלירמאש פתרונות בע"מ -
Overproduction of the β subunit of DNA polymerase III holoenzyme reduces UV mutagenesis in Escherichia coli
Year:
1992
Source of publication :
Journal of Bacteriology
Authors :
תדמור, יעקב
;
.
Volume :
174
Co-Authors:
Tadmor, Y., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Ascarelli-Goell, R., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Skaliter, R., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Livneh, Z., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Facilitators :
From page:
2517
To page:
2524
(
Total pages:
8
)
Abstract:
Overproduction of the β subunit of DNA polymerase III holoenzyme caused a 5- to 10-fold reduction of UV mutagenesis along with a slight increase in sensitivity to UV light in Escherichia coli. The same effects were observed in excision-deficient cells, excluding the possibility that they were mediated via changes in excision repair. In contrast, overproduction of the α subunit of the polymerase did not influence either UV mutagenesis or UV sensitivity. The presence of the mutagenesis proteins MucA and MucB expressed from a plasmid alleviated the effect of overproduced β on UV mutagenesis. We have previously suggested that DNA polymerase III holoenzyme can exist in two forms: β-rich form unable to bypass UV lesions and a β-poor form capable of bypassing UV lesions (O. Shavitt and Z. Livneh, J. Biol. Chem. 264:11275- 11281, 1989). The β-poor form may be related to an SOS form of DNA polymerase III designed to perform translesion polymerization under SOS conditions and thereby generate mutations. On the basis of this model, we propose that the overproduced β subunit affects the relative abundance of the regular replicative β-rich polymerase and the SOS bypass-proficient polymerase by sequestering the polymerase molecules to the β-rich form and blocking the SOS form.
Note:
Related Files :
alpha chain
DNA damage
DNA polymerase III
dna repair
gene expression
Plasmid
stress
ultraviolet radiation
Ultraviolet Rays
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24490
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
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Scientific Publication
Overproduction of the β subunit of DNA polymerase III holoenzyme reduces UV mutagenesis in Escherichia coli
174
Tadmor, Y., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Ascarelli-Goell, R., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Skaliter, R., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Livneh, Z., Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel
Overproduction of the β subunit of DNA polymerase III holoenzyme reduces UV mutagenesis in Escherichia coli
Overproduction of the β subunit of DNA polymerase III holoenzyme caused a 5- to 10-fold reduction of UV mutagenesis along with a slight increase in sensitivity to UV light in Escherichia coli. The same effects were observed in excision-deficient cells, excluding the possibility that they were mediated via changes in excision repair. In contrast, overproduction of the α subunit of the polymerase did not influence either UV mutagenesis or UV sensitivity. The presence of the mutagenesis proteins MucA and MucB expressed from a plasmid alleviated the effect of overproduced β on UV mutagenesis. We have previously suggested that DNA polymerase III holoenzyme can exist in two forms: β-rich form unable to bypass UV lesions and a β-poor form capable of bypassing UV lesions (O. Shavitt and Z. Livneh, J. Biol. Chem. 264:11275- 11281, 1989). The β-poor form may be related to an SOS form of DNA polymerase III designed to perform translesion polymerization under SOS conditions and thereby generate mutations. On the basis of this model, we propose that the overproduced β subunit affects the relative abundance of the regular replicative β-rich polymerase and the SOS bypass-proficient polymerase by sequestering the polymerase molecules to the β-rich form and blocking the SOS form.
Scientific Publication
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