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פותח על ידי קלירמאש פתרונות בע"מ -
Measurement of essential physical properties of vitrification solutions
Year:
2007
Source of publication :
Theriogenology
Authors :
שלום, יבין
;
.
Volume :
67
Co-Authors:
Yavin, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
81
To page:
89
(
Total pages:
9
)
Abstract:
Vitrification is an "ice-free" cryopreservation method that has rapidly developed in recent years and might become the method of choice for oocyte cryopreservation. Five sources of damage should be considered when attempting to achieve successful oocyte cryopreservation by vitrification:1.Solution effects2.Crystallization3.Glass fractures4.Devitrification and recrystallization5.Chilling injury. The probability of successful vitrification depends on three major factors: viscosity of the sample; cooling and warming rates; and sample volume. One of the problems associated with the vitrification solution is that it may contain high concentrations of cryoprotectants (CP), which can damage the cells through chemical toxicity and osmotic shock. In the present study, we examined the principal parameters associated with successful vitrification, and attempted to compose guidelines to the most important aspects of the vitrification process. The first step was the selection of a suitable and least toxic vitrification solution. We then evaluated the effects of cooling rate and volume on the probability of vitrification. Reduction of the sample volume, combined with accelerated cooling, enabled reduction of the CP concentration. However, in practice, a delicate balance must be maintained among all the factors that affect the probability of vitrification in order to prevent crystallization, devitrification, recrystallization, glass fractures and chilling injury. Crown Copyright © 2006.
Note:
Related Files :
Animal
Animals
chilling injury
cryoprotective agent
Cryoprotective Agents
drug effect
Gametes
human
Oocytes
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.theriogenology.2006.09.029
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24599
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
You may also be interested in
Scientific Publication
Measurement of essential physical properties of vitrification solutions
67
Yavin, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Measurement of essential physical properties of vitrification solutions
Vitrification is an "ice-free" cryopreservation method that has rapidly developed in recent years and might become the method of choice for oocyte cryopreservation. Five sources of damage should be considered when attempting to achieve successful oocyte cryopreservation by vitrification:1.Solution effects2.Crystallization3.Glass fractures4.Devitrification and recrystallization5.Chilling injury. The probability of successful vitrification depends on three major factors: viscosity of the sample; cooling and warming rates; and sample volume. One of the problems associated with the vitrification solution is that it may contain high concentrations of cryoprotectants (CP), which can damage the cells through chemical toxicity and osmotic shock. In the present study, we examined the principal parameters associated with successful vitrification, and attempted to compose guidelines to the most important aspects of the vitrification process. The first step was the selection of a suitable and least toxic vitrification solution. We then evaluated the effects of cooling rate and volume on the probability of vitrification. Reduction of the sample volume, combined with accelerated cooling, enabled reduction of the CP concentration. However, in practice, a delicate balance must be maintained among all the factors that affect the probability of vitrification in order to prevent crystallization, devitrification, recrystallization, glass fractures and chilling injury. Crown Copyright © 2006.
Scientific Publication
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