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פותח על ידי קלירמאש פתרונות בע"מ -
The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
Year:
2012
Authors :
מנוליס-ששון, שולמית
;
.
צ'לופוביץ, לאורה
;
.
Volume :
25
Co-Authors:
Nissan, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Yeheskel, A., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Pasmanik-Chor, M., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Facilitators :
From page:
231
To page:
240
(
Total pages:
10
)
Abstract:
The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila. © 2012 The American Phytopathological Society.
Note:
Related Files :
Genetics
Gypsophila
metabolism
Microbiology
molecular genetics
Plant Disease
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-06-11-0173
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24627
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
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Scientific Publication
The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
25
Nissan, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Yeheskel, A., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Pasmanik-Chor, M., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila. © 2012 The American Phytopathological Society.
Scientific Publication
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