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פותח על ידי קלירמאש פתרונות בע"מ -
Cloning of tangerine from tomato reveals a Carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants
Year:
2002
Source of publication :
Plant Cell
Authors :
איזיקסון, טל
;
.
Volume :
14
Co-Authors:
Isaacson, T., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Ronen, G., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Zamir, D., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Hirschberg, J., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Facilitators :
From page:
333
To page:
342
(
Total pages:
10
)
Abstract:
Carotenoid biosynthesis in plants has been described at the molecular level for most of the biochemical steps in the pathway. However, the cis-trans isomerization of carotenoids, which is known to occur in vivo, has remained a mystery since its discovery five decades ago. To elucidate the molecular mechanism of carotenoid isomerization, we have taken a genetic map-based approach to clone the tangerine locus from tomato. Fruit of tangerine are orange and accumulate prolycopene (7Z,9Z,7′Z,9′Z-tetra-cis-lycopene) instead of the all-trans-lycopene, which normally is synthesized in the wild type. Our data indicate that the tangerine gene, designated CRTISO, encodes an authentic carotenoid isomerase that is required during carotenoid desaturation. CRTISO is a redox-type enzyme structurally related to the bacterial-type phytoene desaturase CRTI. Two alleles of tangerine have been investigated. In tangerinemic, loss of function is attributable to a deletion mutation in CRTISO, and in tangerine3183, expression of this gene is impaired. CRTISO from tomato is expressed in all green tissues but is upregulated during fruit ripening and in flowers. The function of carotene isomerase in plants presumably is to enable carotenoid biosynthesis to occur in the dark and in nonphotosynthetic tissues.
Note:
Related Files :
biosynthesis
chromosome mapping
cloning
enzymes
Genetics
metabolism
molecular genetics
mutation
photosynthesis
עוד תגיות
תוכן קשור
More details
DOI :
10.1105/tpc.010303
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24707
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
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Scientific Publication
Cloning of tangerine from tomato reveals a Carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants
14
Isaacson, T., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Ronen, G., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Zamir, D., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Hirschberg, J., Department of Genetics, Hebrew University of Jerusalem, Jerusalem 91904, Israel
Cloning of tangerine from tomato reveals a Carotenoid isomerase essential for the production of β-carotene and xanthophylls in plants
Carotenoid biosynthesis in plants has been described at the molecular level for most of the biochemical steps in the pathway. However, the cis-trans isomerization of carotenoids, which is known to occur in vivo, has remained a mystery since its discovery five decades ago. To elucidate the molecular mechanism of carotenoid isomerization, we have taken a genetic map-based approach to clone the tangerine locus from tomato. Fruit of tangerine are orange and accumulate prolycopene (7Z,9Z,7′Z,9′Z-tetra-cis-lycopene) instead of the all-trans-lycopene, which normally is synthesized in the wild type. Our data indicate that the tangerine gene, designated CRTISO, encodes an authentic carotenoid isomerase that is required during carotenoid desaturation. CRTISO is a redox-type enzyme structurally related to the bacterial-type phytoene desaturase CRTI. Two alleles of tangerine have been investigated. In tangerinemic, loss of function is attributable to a deletion mutation in CRTISO, and in tangerine3183, expression of this gene is impaired. CRTISO from tomato is expressed in all green tissues but is upregulated during fruit ripening and in flowers. The function of carotene isomerase in plants presumably is to enable carotenoid biosynthesis to occur in the dark and in nonphotosynthetic tissues.
Scientific Publication
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