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פותח על ידי קלירמאש פתרונות בע"מ -
Epidemiological study of Cucumber green mottle mosaic virus in greenhouses enables reduction of disease damage in cucurbit production
Year:
2016
Source of publication :
Annals of Applied Biology
Authors :
בלאוסוב, אדוארד
;
.
דומברובסקי, אביב
;
.
לכמן, עודד
;
.
ריינגולד, ויקטוריה
;
.
Volume :
168
Co-Authors:
Reingold, V., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel, Hebrew University of Jerusalem, Genetics and Breeding, Rehovot, Israel
Lachman, O., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel
Belausov, E., Department of Plant Science, ARO, Volcani Center, Beit Dagan, Israel
Koren, A., Hishtil Nursery, Nehalim, Israel
Mor, N., Extension Service, Ministry of Agriculture and Rural Development, Beit Dagan, Israel
Dombrovsky, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel
Facilitators :
From page:
29
To page:
40
(
Total pages:
12
)
Abstract:
Since 2007, the tobamovirus Cucumber green mottle mosaic virus (CGMMV) has become widespread in Israel, causing severe damage to trellised cucumber and melon in greenhouses and watermelon grown in open fields. To reduce disease damage below the economic threshold, this study focused on four objectives: (a) monitoring the patterns of virus distribution within commercial cucumber greenhouses; (b) studying the potential transmission of CGMMV by agrotechnical activities; (c) virus localization in plant tissues; and (d) searching for techniques that might be adapted for mitigating the disease in trellised cucurbit growth. The results of our surveys demonstrated the role of contaminated seeds and soil as primary inoculum sources, and secondary spread caused by agrotechnical activities. The patterns of secondary disease spread were demonstrated in a series of inoculation experiments involving contaminated knives, shears or hands on wet and dry plants, conducted under research-greenhouse conditions. In parallel experiments using CGMMV-specific antibody and secondary antibody conjugated to Alexa fluor 488, the viral coat protein was visualized in several plant tissues: phloem, xylem, trichomes and grasping tendrils. In addition, commercial-greenhouse experiments were aimed at reducing the number of inoculum sources by identifying and removing infected plants from the plots (early monitoring) prior to agrotechnical activities and/or by adding intermediate medium (IM), such as virus-free compost, to the planting pits at the planting stage. It is suggested that the use of IM combined with early monitoring, awareness of worker mobility (from contaminated structures to young planting areas) and proper sanitation (e.g. disinfection of agrotechnical tools) may reduce the yield losses caused by CGMMV below the economic threshold. © 2015 Association of Applied Biologists.
Note:
Related Files :
climbing plant
Cucumis sativus
epidemiology
Growth
Inoculation
Israel
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/aab.12238
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24750
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
Scientific Publication
Epidemiological study of Cucumber green mottle mosaic virus in greenhouses enables reduction of disease damage in cucurbit production
168
Reingold, V., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel, Hebrew University of Jerusalem, Genetics and Breeding, Rehovot, Israel
Lachman, O., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel
Belausov, E., Department of Plant Science, ARO, Volcani Center, Beit Dagan, Israel
Koren, A., Hishtil Nursery, Nehalim, Israel
Mor, N., Extension Service, Ministry of Agriculture and Rural Development, Beit Dagan, Israel
Dombrovsky, A., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Beit Dagan, Israel
Epidemiological study of Cucumber green mottle mosaic virus in greenhouses enables reduction of disease damage in cucurbit production
Since 2007, the tobamovirus Cucumber green mottle mosaic virus (CGMMV) has become widespread in Israel, causing severe damage to trellised cucumber and melon in greenhouses and watermelon grown in open fields. To reduce disease damage below the economic threshold, this study focused on four objectives: (a) monitoring the patterns of virus distribution within commercial cucumber greenhouses; (b) studying the potential transmission of CGMMV by agrotechnical activities; (c) virus localization in plant tissues; and (d) searching for techniques that might be adapted for mitigating the disease in trellised cucurbit growth. The results of our surveys demonstrated the role of contaminated seeds and soil as primary inoculum sources, and secondary spread caused by agrotechnical activities. The patterns of secondary disease spread were demonstrated in a series of inoculation experiments involving contaminated knives, shears or hands on wet and dry plants, conducted under research-greenhouse conditions. In parallel experiments using CGMMV-specific antibody and secondary antibody conjugated to Alexa fluor 488, the viral coat protein was visualized in several plant tissues: phloem, xylem, trichomes and grasping tendrils. In addition, commercial-greenhouse experiments were aimed at reducing the number of inoculum sources by identifying and removing infected plants from the plots (early monitoring) prior to agrotechnical activities and/or by adding intermediate medium (IM), such as virus-free compost, to the planting pits at the planting stage. It is suggested that the use of IM combined with early monitoring, awareness of worker mobility (from contaminated structures to young planting areas) and proper sanitation (e.g. disinfection of agrotechnical tools) may reduce the yield losses caused by CGMMV below the economic threshold. © 2015 Association of Applied Biologists.
Scientific Publication
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