נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection
Year:
2012
Source of publication :
Phytopathology
Authors :
דרור, אורית
;
.
מנוליס-ששון, שולמית
;
.
צ'לופוביץ, לאורה
;
.
Volume :
102
Co-Authors:
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Zellermann, E.-M., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Fluegel, M., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Dror, O., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Eichenlaub, R., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Gartemann, K.-H., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Savidor, A., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Iraki, N., UNESCO Biotechnology Center, Bethlehem University, Bethlehem, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
23
To page:
31
(
Total pages:
9
)
Abstract:
The vascular pathogen Clavibacter michiganensis subsp. michiganensis is responsible for bacterial wilt and canker of tomato. Pathogenicity of this bacterium is dependent on plasmid-borne virulence factors and serine proteases located on the chromosomal chp/tomA pathogenicity island (PAI). In this study, colonization patterns and movement of C. michiganensis subsp. michiganensis during tomato infection was examined using a green fluorescent protein (GFP)-labeled strain. A plasmid expressing GFP in C. michiganensis subsp. michiganensis was constructed and found to be stable in planta for at least 1 month. Confocal laserscanning microscopy (CLSM) of inoculated stems showed that the pathogen extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem. Acropetal movement of the wild-type strain C. michiganensis subsp. michiganensis NCPPB382 (Cmm382) in tomato resulted in an extensive systemic colonization of the whole plant reaching the apical region after 15 days, whereas Cmm100 (lacking the plasmids pCM1 and pCM2) or Cmm27 (lacking the chp/tomA PAI) remained confined to the area surrounding of the inoculation site. Cmm382 formed biofilm-like structures composed of large bacterial aggregates on the interior of xylem walls as observed by CLSM and scanning electron microscopy. These findings suggest that virulence factors located on the chp/tomA PAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates. © 2012 The American Phytopathological Society.
Note:
Related Files :
Chromosomes, Bacterial
Genetics
Growth, Development and Aging
metabolism
Microbiology
Plant Disease
Plasmid
ultrastructure
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24755
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
You may also be interested in
Scientific Publication
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection
102
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Zellermann, E.-M., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Fluegel, M., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Dror, O., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Eichenlaub, R., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Gartemann, K.-H., Department of Microbiology/Genetechnology, Faculty of Biology, University of Bielefeld, Bielefeld, Germany
Savidor, A., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Iraki, N., UNESCO Biotechnology Center, Bethlehem University, Bethlehem, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan, Israel
Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection
The vascular pathogen Clavibacter michiganensis subsp. michiganensis is responsible for bacterial wilt and canker of tomato. Pathogenicity of this bacterium is dependent on plasmid-borne virulence factors and serine proteases located on the chromosomal chp/tomA pathogenicity island (PAI). In this study, colonization patterns and movement of C. michiganensis subsp. michiganensis during tomato infection was examined using a green fluorescent protein (GFP)-labeled strain. A plasmid expressing GFP in C. michiganensis subsp. michiganensis was constructed and found to be stable in planta for at least 1 month. Confocal laserscanning microscopy (CLSM) of inoculated stems showed that the pathogen extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem. Acropetal movement of the wild-type strain C. michiganensis subsp. michiganensis NCPPB382 (Cmm382) in tomato resulted in an extensive systemic colonization of the whole plant reaching the apical region after 15 days, whereas Cmm100 (lacking the plasmids pCM1 and pCM2) or Cmm27 (lacking the chp/tomA PAI) remained confined to the area surrounding of the inoculation site. Cmm382 formed biofilm-like structures composed of large bacterial aggregates on the interior of xylem walls as observed by CLSM and scanning electron microscopy. These findings suggest that virulence factors located on the chp/tomA PAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates. © 2012 The American Phytopathological Society.
Scientific Publication
You may also be interested in