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Cytosolic monoterpene biosynthesis is supported by plastid-generated geranyl diphosphate substrate in transgenic tomato fruits
Year:
2013
Source of publication :
Plant Journal
Authors :
דוידוביץ'-רקנאטי, רחל
;
.
לוינסון, אפרים
;
.
Volume :
75
Co-Authors:
Gutensohn, M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States
Nguyen, T.T.H., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor MI 48109, United States, Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam
Davidovich-Rikanati, R., Department of Vegetable Crops, Newe Yaár Research Center, Agricultural Research Organisation, PO Box 1021, Ramat Yishay, Israel
Ferruzzi, M.G., Department of Food Science, Purdue University, West Lafayette IN 47907, United States
Sitrit, Y., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel
Lewinsohn, E., Department of Vegetable Crops, Newe Yaár Research Center, Agricultural Research Organisation, PO Box 1021, Ramat Yishay, Israel
Pichersky, E., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States, Department of Biochemistry, Purdue University, West Lafayette IN 47907, United States
Facilitators :
From page:
351
To page:
363
(
Total pages:
13
)
Abstract:
Geranyl diphosphate (GPP), the precursor of most monoterpenes, is synthesized in plastids from dimethylallyl diphosphate and isopentenyl diphosphate by GPP synthases (GPPSs). In heterodimeric GPPSs, a non-catalytic small subunit (GPPS-SSU) interacts with a catalytic large subunit, such as geranylgeranyl diphosphate synthase, and determines its product specificity. Here, snapdragon (Antirrhinum majus) GPPS-SSU was over-expressed in tomato fruits under the control of the fruit ripening-specific polygalacturonase promoter to divert the metabolic flux from carotenoid formation towards GPP and monoterpene biosynthesis. Transgenic tomato fruits produced monoterpenes, including geraniol, geranial, neral, citronellol and citronellal, while exhibiting reduced carotenoid content. Co-expression of the Ocimum basilicum geraniol synthase (GES) gene with snapdragon GPPS-SSU led to a more than threefold increase in monoterpene formation in tomato fruits relative to the parental GES line, indicating that the produced GPP can be used by plastidic monoterpene synthases. Co-expression of snapdragon GPPS-SSU with the O. basilicum α-zingiberene synthase (ZIS) gene encoding a cytosolic terpene synthase that has been shown to possess both sesqui- and monoterpene synthase activities resulted in increased levels of ZIS-derived monoterpene products compared to fruits expressing ZIS alone. These results suggest that re-direction of the metabolic flux towards GPP in plastids also increases the cytosolic pool of GPP available for monoterpene synthesis in this compartment via GPP export from plastids. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.
Note:
Related Files :
Antirrhinum
Biochemistry
biosynthesis
gene expression
Genetics
metabolism
Ocimum basilicum
Solanum lycopersicum
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.12212
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24767
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
You may also be interested in
Scientific Publication
Cytosolic monoterpene biosynthesis is supported by plastid-generated geranyl diphosphate substrate in transgenic tomato fruits
75
Gutensohn, M., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States
Orlova, I., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States
Nguyen, T.T.H., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor MI 48109, United States, Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam
Davidovich-Rikanati, R., Department of Vegetable Crops, Newe Yaár Research Center, Agricultural Research Organisation, PO Box 1021, Ramat Yishay, Israel
Ferruzzi, M.G., Department of Food Science, Purdue University, West Lafayette IN 47907, United States
Sitrit, Y., Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel
Lewinsohn, E., Department of Vegetable Crops, Newe Yaár Research Center, Agricultural Research Organisation, PO Box 1021, Ramat Yishay, Israel
Pichersky, E., Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor MI 48109, United States
Dudareva, N., Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette IN 47907, United States, Department of Biochemistry, Purdue University, West Lafayette IN 47907, United States
Cytosolic monoterpene biosynthesis is supported by plastid-generated geranyl diphosphate substrate in transgenic tomato fruits
Geranyl diphosphate (GPP), the precursor of most monoterpenes, is synthesized in plastids from dimethylallyl diphosphate and isopentenyl diphosphate by GPP synthases (GPPSs). In heterodimeric GPPSs, a non-catalytic small subunit (GPPS-SSU) interacts with a catalytic large subunit, such as geranylgeranyl diphosphate synthase, and determines its product specificity. Here, snapdragon (Antirrhinum majus) GPPS-SSU was over-expressed in tomato fruits under the control of the fruit ripening-specific polygalacturonase promoter to divert the metabolic flux from carotenoid formation towards GPP and monoterpene biosynthesis. Transgenic tomato fruits produced monoterpenes, including geraniol, geranial, neral, citronellol and citronellal, while exhibiting reduced carotenoid content. Co-expression of the Ocimum basilicum geraniol synthase (GES) gene with snapdragon GPPS-SSU led to a more than threefold increase in monoterpene formation in tomato fruits relative to the parental GES line, indicating that the produced GPP can be used by plastidic monoterpene synthases. Co-expression of snapdragon GPPS-SSU with the O. basilicum α-zingiberene synthase (ZIS) gene encoding a cytosolic terpene synthase that has been shown to possess both sesqui- and monoterpene synthase activities resulted in increased levels of ZIS-derived monoterpene products compared to fruits expressing ZIS alone. These results suggest that re-direction of the metabolic flux towards GPP in plastids also increases the cytosolic pool of GPP available for monoterpene synthesis in this compartment via GPP export from plastids. © 2013 The Authors The Plant Journal © 2013 John Wiley & Sons Ltd.
Scientific Publication
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