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פותח על ידי קלירמאש פתרונות בע"מ -
Cloning and characterization of the tomato karyopherin α1 gene promoter
Year:
2004
Authors :
גפני, ידידיה
;
.
דבוש, דוד
;
.
לוי, יעל
;
.
Volume :
46
Co-Authors:
Mizrachy, L., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dabush, D., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Levy, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Aloni, R., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Altman, A., R. H. S. Inst. Plant Sci./Genet. A., Hebrew University of Jerusalem, Rehovot 76100, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
515
To page:
522
(
Total pages:
8
)
Abstract:
The karyopherin α1 (LeKAPα 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1-GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPα 1 expression.
Note:
Related Files :
Base Sequence
gene expression
Genome
nuclear localization signal
Petunia
plant growth
Sequence Deletion
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1440-169x.2004.00766.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24782
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
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Scientific Publication
Cloning and characterization of the tomato karyopherin α1 gene promoter
46
Mizrachy, L., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Dabush, D., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Levy, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Aloni, R., Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Altman, A., R. H. S. Inst. Plant Sci./Genet. A., Hebrew University of Jerusalem, Rehovot 76100, Israel
Gafni, Y., Department of Genetics, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Cloning and characterization of the tomato karyopherin α1 gene promoter
The karyopherin α1 (LeKAPα 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1-GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPα 1 expression.
Scientific Publication
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