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פותח על ידי קלירמאש פתרונות בע"מ -
Genetic diversity, anastomosis groups and virulence of Rhizoctonia spp. from strawberry
Year:
2007
Authors :
פרימן, סטנלי
;
.
שרון, מיכל
;
.
Volume :
117
Co-Authors:
Sharon, M., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Freeman, S., Department of Plant Pathology, Agriculture Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kuninaga, S., Department of Integrated Human Sciences, School of Dentistry, Health Sciences University of Hokkaido, Tohbetsu, Hokkaido 061-0293, Japan
Sneh, B., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Facilitators :
From page:
247
To page:
265
(
Total pages:
19
)
Abstract:
Virulent Rhizoctonia spp. isolated from strawberry in Israel belonged to anastomosis groups (AG) of: binucleate Rhizoctonia (BNR) AG-A, AG-G, AG-K and AG-F, and to multinucleate Rhizoctonia (MNR) AG 4 subgroup HG-I. In addition, a soil isolate of AG 4 subgroup HG-III was also found to be virulent on strawberry. None of the Israeli isolates obtained in the present study belonged to BNR AG-I, or other MNR AGs. In the cluster analysis of rDNA-ITS sequences, all of the isolate sequences consistently clustered according to their known AGs and subgroups. One AG-F cluster included sequences of 10 strawberry isolates, while another AG-F cluster included sequences of two isolates submitted to GenBank. Additional work is needed to determine whether the isolates of these two clusters may belong to different AG-F subgroups. The current virulence bioassay used for Rhizoctonia spp. isolates on strawberry is based on inoculation of stolon-derived daughter plants with the isolates and estimation of the reduction in plant biomass, rather than on specific distinct disease severity symptoms. The duration of this test is relatively long (ca. 5 weeks or more) and the availability of daughter plants from runners is naturally limited to a certain season. Among the possible alternative methods evaluated in the present study (inoculation of fruits or seedlings developed from germinated strawberry seeds), the method based on seedlings was best. This method has a potential to replace the currently used stolon-daughter plant inoculation bioassay for testing virulence of strawberry root pathogens. This is the first report indicating that Rhizoctonia spp. isolates that belong to AG-F, AG-K, AG 4 HG-I and AG 4 HG-III are virulent to strawberry. © 2007 KNPV.
Note:
Related Files :
Bioassay
Fragaria ananassa
host-pathogen interaction
Inoculation
Israel
Percent sequence similarity
Rhizoctonia
shrub
virulence
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s10658-006-9091-7
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
24799
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:10
Scientific Publication
Genetic diversity, anastomosis groups and virulence of Rhizoctonia spp. from strawberry
117
Sharon, M., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Freeman, S., Department of Plant Pathology, Agriculture Research Organization, Volcani Center, Bet Dagan 50250, Israel
Kuninaga, S., Department of Integrated Human Sciences, School of Dentistry, Health Sciences University of Hokkaido, Tohbetsu, Hokkaido 061-0293, Japan
Sneh, B., Department of Plant Sciences, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
Genetic diversity, anastomosis groups and virulence of Rhizoctonia spp. from strawberry
Virulent Rhizoctonia spp. isolated from strawberry in Israel belonged to anastomosis groups (AG) of: binucleate Rhizoctonia (BNR) AG-A, AG-G, AG-K and AG-F, and to multinucleate Rhizoctonia (MNR) AG 4 subgroup HG-I. In addition, a soil isolate of AG 4 subgroup HG-III was also found to be virulent on strawberry. None of the Israeli isolates obtained in the present study belonged to BNR AG-I, or other MNR AGs. In the cluster analysis of rDNA-ITS sequences, all of the isolate sequences consistently clustered according to their known AGs and subgroups. One AG-F cluster included sequences of 10 strawberry isolates, while another AG-F cluster included sequences of two isolates submitted to GenBank. Additional work is needed to determine whether the isolates of these two clusters may belong to different AG-F subgroups. The current virulence bioassay used for Rhizoctonia spp. isolates on strawberry is based on inoculation of stolon-derived daughter plants with the isolates and estimation of the reduction in plant biomass, rather than on specific distinct disease severity symptoms. The duration of this test is relatively long (ca. 5 weeks or more) and the availability of daughter plants from runners is naturally limited to a certain season. Among the possible alternative methods evaluated in the present study (inoculation of fruits or seedlings developed from germinated strawberry seeds), the method based on seedlings was best. This method has a potential to replace the currently used stolon-daughter plant inoculation bioassay for testing virulence of strawberry root pathogens. This is the first report indicating that Rhizoctonia spp. isolates that belong to AG-F, AG-K, AG 4 HG-I and AG 4 HG-III are virulent to strawberry. © 2007 KNPV.
Scientific Publication
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